Spinal Segment Range of Motion As a Function of in Vitro Test Conditions: Effects of Exposure Period, Accumulated Cycles, Angular-deformation Rate, and Moisture Condition

Background: The purpose of this study was to thoroughly evaluate the relationship of possible mechanical and environmental conditions in the testing of spinal joint segments, including ambient-temperature exposure, accumulated test cycles, angular-deformation rate, and moisture condition on the motion characteristics of joints, with the example of isolated spinal segments.

Methods: In one test, controlled loading was applied to six motion segments every 8 hours in each of the primary anatomical directions while moisture was held constant. In a second test, 18 specimens were divided equally into moisture-static, air-exposed, and slowly irrigated groups and loaded to 500 cycles. In a third test, a similar sample was tested over a range of angular-deformation rates (0.6-5.1 degrees/second).

Results: Range of motion (ROM) increased steadily with ambient-temperature exposure time, resulting in a 10% change at about 20 hours but was most stable in the moisture-static group over both accumulated load cycles and loading rate changes.

Conclusions: The most critical factor for functional testing of spinal segments appears to be length of exposure.