Isolation of the Ornithine-delta-aminotransferase CDNA and Effect of Salt Stress on Its Expression in Arabidopsis Thaliana

Overview

Journal Plant Physiol

Specialty Physiology

Date 1998 May 22

PMID 9576796

Citations 51

Authors

N H Roosens

T T Thu

H M Iskandar

M Jacobs

Affiliations

Soon will be listed here.

Abstract

To evaluate the relative importance of ornithine (Orn) as a precursor in proline (Pro) synthesis, we isolated and sequenced a cDNA encoding the Orn-delta-aminotransferase (delta-OAT) from Arabidopsis thaliana. The deduced amino acid sequence showed high homology with bacterial, yeast, mammalian, and plant sequences, and the N-terminal residues exhibited several common features with a mitochondrial transit peptide. Our results show that under both salt stress and normal conditions, delta-OAT activity and mRNA in young plantlets are slightly higher than in older plants. This appears to be related to the necessity to dispose of an easy recycling product, glutamate. Analysis of the expression of the gene revealed a close association with salt stress and Pro production. In young plantlets, free Pro content, Delta1-pyrroline-5-carboxylate synthase mRNA, delta-OAT activity, and delta-OAT mRNA were all increased by salt-stress treatment. These results suggest that for A. thaliana, the Orn pathway, together with the glutamate pathway, plays an important role in Pro accumulation during osmotic stress. Conversely, in 4-week-old A. thaliana plants, although free Pro level also increased under salt-stress conditions, the delta-OAT activity appeared to be unchanged and delta-OAT mRNA was not detectable. Delta1-pyrroline-5-carboxylate synthase mRNA was still induced at a similar level. Therefore, for the adult plants the free Pro increase seemed to be due to the activity of the enzymes of the glutamate pathway.

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References

von Heijne G, Steppuhn J, Herrmann R . Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem. 1989; 180(3):535-45. DOI: 10.1111/j.1432-1033.1989.tb14679.x. View

Davis R . Compartmental and regulatory mechanisms in the arginine pathways of Neurospora crassa and Saccharomyces cerevisiae. Microbiol Rev. 1986; 50(3):280-313. PMC: 373072. DOI: 10.1128/mr.50.3.280-313.1986. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Hu C, Delauney A, Verma D . A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants. Proc Natl Acad Sci U S A. 1992; 89(19):9354-8. PMC: 50125. DOI: 10.1073/pnas.89.19.9354. View

Gardan R, Rapoport G, Debarbouille M . Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis. J Mol Biol. 1995; 249(5):843-56. DOI: 10.1006/jmbi.1995.0342. View

Canel C, Roose M . Molecular characterization of the mitochondrial citrate synthase gene of an acidless pummelo (Citrus maxima). Plant Mol Biol. 1996; 31(1):143-7. DOI: 10.1007/BF00020613. View

Whatmore A, Chudek J, Reed R . The effects of osmotic upshock on the intracellular solute pools of Bacillus subtilis. J Gen Microbiol. 1990; 136(12):2527-35. DOI: 10.1099/00221287-136-12-2527. View

Boggess S . Contribution of Arginine to Proline Accumulation in Water-stressed Barley Leaves. Plant Physiol. 1976; 58(6):796-7. PMC: 542311. DOI: 10.1104/pp.58.6.796. View

Schultz C, Coruzzi G . The aspartate aminotransferase gene family of Arabidopsis encodes isoenzymes localized to three distinct subcellular compartments. Plant J. 1995; 7(1):61-75. DOI: 10.1046/j.1365-313x.1995.07010061.x. View

10.

Boggess S, Stewart C . Effect of water stress on proline synthesis from radioactive precursors. Plant Physiol. 1976; 58(3):398-401. PMC: 542254. DOI: 10.1104/pp.58.3.398. View

11.

Csonka L . Physiological and genetic responses of bacteria to osmotic stress. Microbiol Rev. 1989; 53(1):121-47. PMC: 372720. DOI: 10.1128/mr.53.1.121-147.1989. View

12.

Heimberg H, Boyen A, Crabeel M, Glansdorff N . Escherichia coli and Saccharomyces cerevisiae acetylornithine aminotransferase: evolutionary relationship with ornithine aminotransferase. Gene. 1990; 90(1):69-78. DOI: 10.1016/0378-1119(90)90440-3. View

13.

Rerie W, Whitecross M, Higgins T . Developmental and environmental regulation of pea legumin genes in transgenic tobacco. Mol Gen Genet. 1991; 225(1):148-57. DOI: 10.1007/BF00282653. View

14.

Cunin R, Glansdorff N, PIERARD A, Stalon V . Biosynthesis and metabolism of arginine in bacteria. Microbiol Rev. 1986; 50(3):314-52. PMC: 373073. DOI: 10.1128/mr.50.3.314-352.1986. View

15.

Savoure A, Jaoua S, Hua X, Ardiles W, Van Montagu M, Verbruggen N . Isolation, characterization, and chromosomal location of a gene encoding the delta 1-pyrroline-5-carboxylate synthetase in Arabidopsis thaliana. FEBS Lett. 1995; 372(1):13-9. DOI: 10.1016/0014-5793(95)00935-3. View

16.

Zonia L, Stebbins N, Polacco J . Essential role of urease in germination of nitrogen-limited Arabidopsis thaliana seeds. Plant Physiol. 1995; 107(4):1097-103. PMC: 157242. DOI: 10.1104/pp.107.4.1097. View

17.

Mestichelli L, Gupta R, SPENSER I . The biosynthetic route from ornithine to proline. J Biol Chem. 1979; 254(3):640-7. View

18.

Yoshiba Y, Kiyosue T, Katagiri T, Ueda H, Mizoguchi T, Yamaguchi-Shinozaki K . Correlation between the induction of a gene for delta 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress. Plant J. 1995; 7(5):751-60. DOI: 10.1046/j.1365-313x.1995.07050751.x. View

19.

Degols G . Functional analysis of the regulatory region adjacent to the cargB gene of Saccharomyces cerevisiae. Nucleotide sequence, gene fusion experiments and cis-dominant regulatory mutation analysis. Eur J Biochem. 1987; 169(1):193-200. DOI: 10.1111/j.1432-1033.1987.tb13597.x. View

20.

Cornish-Bowden A, Eisenthal R . Estimation of Michaelis constant and maximum velocity from the direct linear plot. Biochim Biophys Acta. 1978; 523(1):268-72. DOI: 10.1016/0005-2744(78)90030-x. View