PX, the HBV-encoded Coactivator, Suppresses the Phenotypes of TBP and TAFII250 Mutants

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 1998 May 30

PMID 9553050

Citations 11

Authors

I Haviv

Y Matza

Y Shaul

Affiliations

Soon will be listed here.

Abstract

Hepatitis B virus (HBV) infects humans and causes a wide range of clinical manifestations, from acute hepatitis to hepatocellular carcinoma (HCC). The HBV genome contains multiple promoters with gene expression regulated predominantly by the cellular transcription initiation machinery. Accordingly, the HBV-encoded pX, the only known viral regulator, is a potent transcription coactivator. We investigated the relationship between pX and cellular coactivators. We show that pX restores wild-type activity to inactive TBPAS mutants with poor TAFII250 and activator-binding activity. This pX-mediated recovery, however, is not obtained with inactive TBPAS mutants in binding of other general transcription factors. Remarkably, ts13, a cell line temperature sensitive for TAFII250 function, exhibiting growth arrest and apoptosis at the restrictive temperature, is rescued partially by pX expression, thus generating a pX-dependent cell growth. Collectively, our results suggest that pX suppresses some of the phenotypes of TBP and TAFII250 mutations, implying that pX circumvents the need for a holo-TFIID complex for transcription activation to proceed.

Citing Articles

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Heterogeneous nuclear ribonucleoprotein R enhances transcription from the naturally configured c-fos promoter in vitro.

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Cytochrome C oxidase III interacts with hepatitis B virus X protein in vivo by yeast two-hybrid system.

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Direct interaction of NRSF with TBP: chromatin reorganization and core promoter repression for neuron-specific gene transcription.

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Expression of nuclear factor-kappa B in hepatocellular carcinoma and its relation with the X protein of hepatitis B virus.

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