Structure of Wild-type Yeast RNA Polymerase II and Location of Rpb4 and Rpb7

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1998 May 26

PMID 9545247

Citations 25

Authors

G J Jensen

G Meredith

D A Bushnell

R D Kornberg

Affiliations

Soon will be listed here.

Abstract

The three-dimensional structure of wild-type yeast RNA polymerase II has been determined at a nominal resolution of 24 A. A difference map between this structure and that of the polymerase lacking subunits Rpb4 and Rpb7 showed these two subunits forming part of the floor of the DNA-binding (active center) cleft, and revealed a slight inward movement of the protein domain surrounding the cleft. Surface plasmon resonance measurements showed that Rpb4 and Rpb7 stabilize a minimal pre-initiation complex containing promoter DNA, TATA box-binding protein (TBP), transcription factor TFIIB and the polymerase. These findings suggest that Rpb4 and Rpb7 play a role in coupling the entry of DNA into the active center cleft to closure of the cleft. Such a role can explain why these subunits are necessary for promoter-specific transcription in vitro and for a normal stress response in vivo.

Citing Articles

Dissociation of Rpb4 from RNA polymerase II is important for yeast functionality.

Duek L, Barkai O, Elran R, Adawi I, Choder M PLoS One. 2018; 13(10):e0206161.

PMID: 30359412 PMC: 6201915. DOI: 10.1371/journal.pone.0206161.

Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation.

Allepuz-Fuster P, Martinez-Fernandez V, Garrido-Godino A, Alonso-Aguado S, Hanes S, Navarro F Nucleic Acids Res. 2014; 42(22):13674-88.

PMID: 25416796 PMC: 4267648. DOI: 10.1093/nar/gku1227.

The Rpb4/7 module of RNA polymerase II is required for carbon catabolite repressor protein 4-negative on TATA (Ccr4-not) complex to promote elongation.

Babbarwal V, Fu J, Reese J J Biol Chem. 2014; 289(48):33125-30.

PMID: 25315781 PMC: 4246073. DOI: 10.1074/jbc.C114.601088.

Correct assembly of RNA polymerase II depends on the foot domain and is required for multiple steps of transcription in Saccharomyces cerevisiae.

Garrido-Godino A, Garcia-Lopez M, Navarro F Mol Cell Biol. 2013; 33(18):3611-26.

PMID: 23836886 PMC: 3753863. DOI: 10.1128/MCB.00262-13.

The RNA Pol II sub-complex hsRpb4/7 is required for viability of multiple human cell lines.

Zhao Y, Li K, Ng K, Ng C, Lee K Protein Cell. 2012; 3(11):846-54.

PMID: 23073835 PMC: 4875460. DOI: 10.1007/s13238-012-2085-7.

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