A Novel Method for Transfer of Two-dimensional Crystals from the Air/water Interface to Specimen Grids. EM Sample Preparation/lipid-layer Crystallization

Overview

Journal J Struct Biol

Specialties Cell Biology
Molecular Biology

Date 1995 Jan 1

PMID 7772418

Citations 6

Authors

F J Asturias

R D Kornberg

Affiliations

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Abstract

Transfer of two-dimensional (2-D) crystals formed on lipid layers by suspension from a wire loop is described. This method gives better recovery and better preservation of 2-D crystals than attained in the past. The method has been applied to crystals of yeast RNA polymerase II to enable their analysis in the frozen hydrated state.

Citing Articles

Lipid monolayer and sparse matrix screening for growing two-dimensional crystals for electron crystallography: methods and examples.

Yeager M, Dryden K, Ganser-Pornillos B Methods Mol Biol. 2012; 955:527-37.

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The use of trehalose in the preparation of specimens for molecular electron microscopy.

Chiu P, Kelly D, Walz T Micron. 2011; 42(8):762-72.

PMID: 21752659 PMC: 3156378. DOI: 10.1016/j.micron.2011.06.005.

7A projection map of the S-layer protein sbpA obtained with trehalose-embedded monolayer crystals.

Norville J, Kelly D, Knight Jr T, Belcher A, Walz T J Struct Biol. 2007; 160(3):313-23.

PMID: 17638580 PMC: 2149845. DOI: 10.1016/j.jsb.2007.06.002.

On the freezing and identification of lipid monolayer 2-D arrays for cryoelectron microscopy.

Taylor D, Kelly D, Cheng A, Taylor K J Struct Biol. 2007; 160(3):305-12.

PMID: 17561414 PMC: 2268103. DOI: 10.1016/j.jsb.2007.04.011.

Structure of DNA-dependent protein kinase: implications for its regulation by DNA.

Leuther K, Hammarsten O, Kornberg R, Chu G EMBO J. 1999; 18(5):1114-23.

PMID: 10064579 PMC: 1171203. DOI: 10.1093/emboj/18.5.1114.