High-level Expression of a Viscotoxin in Arabidopsis Thaliana Gives Enhanced Resistance Against Plasmodiophora Brassicae

Overview

Journal Plant Mol Biol

Date 1998 Apr 4

PMID 9526499

Citations 6

Authors

S Holtorf

J Ludwig-Muller

K Apel

H Bohlmann

Affiliations

Soon will be listed here.

Abstract

Viscotoxins are a group of toxic thionins found in several mistletoe species. The constitutive CaMV-omega promoter was used to drive the expression of the viscotoxin A3 cDNA from Viscum album in transgenic Arabidopsis thaliana C24. Lines with high viscotoxin A3 levels in all parts of the plant were selected and tested for resistance against the clubroot pathogen Plasmodiophora brassicae. The transgenic lines were more resistant to infection by this pathogen than the parental line.

Citing Articles

Current Status of the Disease-Resistant Gene(s)/QTLs, and Strategies for Improvement in .

Singh K, Kumari P, Rai P Front Plant Sci. 2021; 12:617405.

PMID: 33747001 PMC: 7965955. DOI: 10.3389/fpls.2021.617405.

The role of thionins in rice defence against root pathogens.

Ji H, Gheysen G, Ullah C, Verbeek R, Shang C, De Vleesschauwer D Mol Plant Pathol. 2015; 16(8):870-81.

PMID: 25676661 PMC: 6638518. DOI: 10.1111/mpp.12246.

Production of an engineered killer peptide in Nicotiana benthamiana by using a potato virus X expression system.

Donini M, Lico C, Baschieri S, Conti S, Magliani W, Polonelli L Appl Environ Microbiol. 2005; 71(10):6360-7.

PMID: 16204558 PMC: 1265961. DOI: 10.1128/AEM.71.10.6360-6367.2005.

Transgenic tomato plants expressing an Arabidopsis thionin (Thi2.1) driven by fruit-inactive promoter battle against phytopathogenic attack.

Chan Y, Prasad V, Sanjaya , Chen K, Liu P, Chan M Planta. 2005; 221(3):386-93.

PMID: 15657715 DOI: 10.1007/s00425-004-1459-3.

Interaction of viscotoxins A3 and B with membrane model systems: implications to their mechanism of action.

Giudici M, Pascual R, de la Canal L, Pfuller K, Pfuller U, Villalain J Biophys J. 2003; 85(2):971-81.

PMID: 12885644 PMC: 1303218. DOI: 10.1016/S0006-3495(03)74536-6.

References

Alexander D, Goodman R, Gut-Rella M, Glascock C, Weymann K, Friedrich L . Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a. Proc Natl Acad Sci U S A. 1993; 90(15):7327-31. PMC: 47130. DOI: 10.1073/pnas.90.15.7327. View

Bohl S, Apel K . A novel fingerprint method for analyzing the expression of complex multigene families of very low transcript abundance. Plant J. 1993; 3(6):887-93. DOI: 10.1111/j.1365-313x.1993.00887.x. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Apel K . cDNA-derived identification of novel thionin precursors in Viscum album that contain highly divergent thionin domains but conserved signal and acidic polypeptide domains. Plant Mol Biol. 1993; 23(6):1233-42. DOI: 10.1007/BF00042356. View

Epple P, Apel K, Bohlmann H . An Arabidopsis thaliana thionin gene is inducible via a signal transduction pathway different from that for pathogenesis-related proteins. Plant Physiol. 1995; 109(3):813-20. PMC: 161381. DOI: 10.1104/pp.109.3.813. View

Melzer S, Majewski D, Apel K . Early Changes in Gene Expression during the Transition from Vegetative to Generative Growth in the Long-Day Plant Sinapis alba. Plant Cell. 1990; 2(10):953-961. PMC: 159944. DOI: 10.1105/tpc.2.10.953. View

SELAWRY O, VESTER F, Mai W, Schwartz M . [On the identification of the constituents of Viscum album. II. Tumor inhibiting substances]. Hoppe Seylers Z Physiol Chem. 1961; 324:262-81. DOI: 10.1515/bchm2.1961.324.1.262. View

Carrasco L, Vazquez D, Hernandez-Lucas C, Carbonero P, Garcia-Olmedo F . Thionins: plant peptides that modify membrane permeability in cultured mammalian cells. Eur J Biochem. 1981; 116(1):185-9. DOI: 10.1111/j.1432-1033.1981.tb05317.x. View

Reimann-Philipp U, Schrader G, Martinoia E, Barkholt V, Apel K . Intracellular thionins of barley. A second group of leaf thionins closely related to but distinct from cell wall-bound thionins. J Biol Chem. 1989; 264(15):8978-84. View

10.

Yang H, McLeese J, Weisbart M, Dionne J, Lemaire I, Aubin R . Simplified high throughput protocol for northern hybridization. Nucleic Acids Res. 1993; 21(14):3337-8. PMC: 309787. DOI: 10.1093/nar/21.14.3337. View

11.

Terras F, Eggermont K, Kovaleva V, Raikhel N, OSBORN R, Kester A . Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell. 1995; 7(5):573-88. PMC: 160805. DOI: 10.1105/tpc.7.5.573. View

12.

Florack D, Dirkse W, Visser B, Heidekamp F, Stiekema W . Expression of biologically active hordothionins in tobacco. Effects of pre- and pro-sequences at the amino and carboxyl termini of the hordothionin precursor on mature protein expression and sorting. Plant Mol Biol. 1994; 24(1):83-96. DOI: 10.1007/BF00040576. View

13.

Schagger H, von Jagow G . Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987; 166(2):368-79. DOI: 10.1016/0003-2697(87)90587-2. View

14.

Samuelsson G, Seger L, Olson T . The amino acid sequence of oxidized viscotoxin A3 from the European mistletoe (Viscum album L, Loranthaceae). Acta Chem Scand. 1968; 22(8):2624-42. DOI: 10.3891/acta.chem.scand.22-2624. View

15.

Holsters M, De Waele D, Depicker A, Messens E, Van Montagu M, Schell J . Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet. 1978; 163(2):181-7. DOI: 10.1007/BF00267408. View

16.

Fernandez de Caleya R, Garcia-Olmedo F, Carbonero P . Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro. Appl Microbiol. 1972; 23(5):998-1000. PMC: 380489. DOI: 10.1128/am.23.5.998-1000.1972. View

17.

Garcia-Olmedo F, Molina A, Segura A, Moreno M . The defensive role of nonspecific lipid-transfer proteins in plants. Trends Microbiol. 1995; 3(2):72-4. DOI: 10.1016/s0966-842x(00)88879-4. View

18.

Steinmuller K, Batschauer A, Apel K . Tissue-specific and light-dependent changes of chromatin organization in barley (Hordeum vulgare). Eur J Biochem. 1986; 158(3):519-25. DOI: 10.1111/j.1432-1033.1986.tb09785.x. View

19.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

20.

Terras F, Schoofs H, Thevissen K, OSBORN R, Vanderleyden J, Cammue B . Synergistic Enhancement of the Antifungal Activity of Wheat and Barley Thionins by Radish and Oilseed Rape 2S Albumins and by Barley Trypsin Inhibitors. Plant Physiol. 1993; 103(4):1311-1319. PMC: 159121. DOI: 10.1104/pp.103.4.1311. View