Stepwise Dynamics of Connecting Filaments Measured in Single Myofibrillar Sarcomeres

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1998 Mar 25

PMID 9512043

Citations 7

Authors

P Yang

T Tameyasu

G H Pollack

Affiliations

Soon will be listed here.

Abstract

Single relaxed myofibrils of bumblebee flight muscle were subjected to motor-imposed ramp-length changes. The image of the striations was projected onto a linear photodiode array, and sarcomere length was computed as the spacing between centroids of contiguous A-bands. Centroid position was determined by integrating the respective A-band intensity peak and computing the location at which the area on one side was equal to the other. The resulting trace of centroid to centroid span versus time was stepwise, with periods of rapid shortening alternating with periods of pause. An alternative nondiscrete sensor gave similar steps. If thick filament length remains constant, stepwise sarcomere length changes imply that length changes in the connecting filament must be stepwise. Thus, shortening of the connecting filament occurs as a sequence of discrete events rather than as a continuous event.

Citing Articles

Sarcomere Lengths Become More Uniform Over Time in Intact Muscle-Tendon Unit During Isometric Contractions.

Kuan Moo E, Herzog W Front Physiol. 2020; 11:448.

PMID: 32477162 PMC: 7235410. DOI: 10.3389/fphys.2020.00448.

Connecting filament mechanics in the relaxed sarcomere.

Nagornyak E, Pollack G J Muscle Res Cell Motil. 2006; 26(6-8):303-6.

PMID: 16453159 DOI: 10.1007/s10974-005-9036-3.

Stepwise length changes in single invertebrate thick filaments.

Nagornyak E, Blyakhman F, Pollack G Biophys J. 2005; 89(5):3269-76.

PMID: 16113114 PMC: 1366822. DOI: 10.1529/biophysj.105.069864.

Effect of sarcomere length on step size in relaxed rabbit psoas muscle.

Nagornyak E, Blyakhman F, Pollack G J Muscle Res Cell Motil. 2004; 25(1):37-43.

PMID: 15160486 DOI: 10.1023/b:jure.0000021375.57900.0d.

Stepwise sliding of single actin and Myosin filaments.

Liu X, Pollack G Biophys J. 2003; 86(1 Pt 1):353-8.

PMID: 14695277 PMC: 1303800. DOI: 10.1016/S0006-3495(04)74111-9.

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