Identification and Localization of High Molecular Weight Proteins in Insect Flight and Leg Muscle

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1990 Nov 1

PMID 2209553

Citations 45

Authors

A Lakey

C Ferguson

S Labeit

M Reedy

A Larkins

G Butcher

K Leonard

B Bullard

Affiliations

Soon will be listed here.

Abstract

Thick and thin filaments in asynchronous flight muscle overlap nearly completely and thick filaments are attached to the Z-disc by connecting filaments. We have raised antibodies against a fraction of Lethocerus flight muscle myofibrils containing Z-discs and associated filaments and also against a low ionic strength extract of myofibrils. Monoclonal antibodies were obtained to proteins of 800 kd (p800), 700 kd (p700), 400 kd (p400) and alpha-actinin. The positions of the proteins in Lethocerus flight and leg myofibrils were determined by immunofluorescence and electron microscopy. p800 is in connecting filaments of flight myofibrils and in A-bands of leg myofibrils. p700 is in Z-discs of flight myofibrils and an immunologically related protein, p500, is in leg muscle Z-discs. p400 is in M-lines of both flight and leg myofibrils. Preliminary DNA sequencing shows that p800 is related to vertebrate titin and nematode twitchin. Molecules of p800 could extend from the Z-disc a short way along thick filaments, forming a mechanical link between the two structures. All three high molecular weight proteins probably stabilize the structure of the myofibril.

Citing Articles

Structure of the Flight Muscle Myosin Filament at 4.7 Å Resolution Reveals New Details of Non-Myosin Proteins.

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A nanobody toolbox to investigate localisation and dynamics of titins and other key sarcomeric proteins.

Loreau V, Rees R, Chan E, Taxer W, Gregor K, Mussil B Elife. 2023; 12.

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Gong H, Ma W, Chen S, Wang G, Khairallah R, Irving T Int J Mol Sci. 2020; 21(15).

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CryoEM structure of flight muscle thick filaments at 7 Å resolution.

Daneshparvar N, Taylor D, OLeary T, Rahmani H, Abbasiyeganeh F, Previs M Life Sci Alliance. 2020; 3(8).

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Nanoscopy reveals the layered organization of the sarcomeric H-zone and I-band complexes.

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References

Pringle J . The Croonian Lecture, 1977. Stretch activation of muscle: function and mechanism. Proc R Soc Lond B Biol Sci. 1978; 201(1143):107-30. DOI: 10.1098/rspb.1978.0035. View

Lambin P . Reliability of molecular weight determination of proteins by polyacrylamide gradient gel electrophoresis in the presence of sodium dodecyl sulfate. Anal Biochem. 1978; 85(1):114-25. DOI: 10.1016/0003-2697(78)90281-6. View

Wang K, McClure J, Tu A . Titin: major myofibrillar components of striated muscle. Proc Natl Acad Sci U S A. 1979; 76(8):3698-702. PMC: 383900. DOI: 10.1073/pnas.76.8.3698. View

Wang K, Williamson C . Identification of an N2 line protein of striated muscle. Proc Natl Acad Sci U S A. 1980; 77(6):3254-8. PMC: 349593. DOI: 10.1073/pnas.77.6.3254. View

Tokuyasu K . Immunochemistry on ultrathin frozen sections. Histochem J. 1980; 12(4):381-403. DOI: 10.1007/BF01011956. View

Sainsbury G, Bullard B . New proline-rich proteins in isolated insect Z-discs. Biochem J. 1980; 191(2):333-9. PMC: 1162222. DOI: 10.1042/bj1910333. View

Saide J . Identification of a connecting filament protein in insect fibrillar flight muscle. J Mol Biol. 1981; 153(3):661-79. DOI: 10.1016/0022-2836(81)90412-5. View

Knight P, Trinick J . Preparation of myofibrils. Methods Enzymol. 1982; 85 Pt B:9-12. DOI: 10.1016/0076-6879(82)85004-0. View

Freundlich A, Squire J . Three-dimensional structure of the insect (Lethocerus) flight muscle M-band. J Mol Biol. 1983; 169(2):439-53. DOI: 10.1016/s0022-2836(83)80060-6. View

10.

White D . The elasticity of relaxed insect fibrillar flight muscle. J Physiol. 1983; 343:31-57. PMC: 1193907. DOI: 10.1113/jphysiol.1983.sp014880. View

11.

Griffiths G, Simons K, Warren G, Tokuyasu K . Immunoelectron microscopy using thin, frozen sections: application to studies of the intracellular transport of Semliki Forest virus spike glycoproteins. Methods Enzymol. 1983; 96:466-85. DOI: 10.1016/s0076-6879(83)96041-x. View

12.

Trinick J, Knight P, Whiting A . Purification and properties of native titin. J Mol Biol. 1984; 180(2):331-56. DOI: 10.1016/s0022-2836(84)80007-8. View

13.

Bullard B, Bell J, Craig R, Leonard K . Arthrin: a new actin-like protein in insect flight muscle. J Mol Biol. 1985; 182(3):443-54. DOI: 10.1016/0022-2836(85)90203-7. View

14.

Francis G, Waterston R . Muscle organization in Caenorhabditis elegans: localization of proteins implicated in thin filament attachment and I-band organization. J Cell Biol. 1985; 101(4):1532-49. PMC: 2113919. DOI: 10.1083/jcb.101.4.1532. View

15.

Reedy M, Reedy M . Rigor crossbridge structure in tilted single filament layers and flared-X formations from insect flight muscle. J Mol Biol. 1985; 185(1):145-76. DOI: 10.1016/0022-2836(85)90188-3. View

16.

Griffiths G, McDOWALL A, BACK R, Dubochet J . On the preparation of cryosections for immunocytochemistry. J Ultrastruct Res. 1984; 89(1):65-78. DOI: 10.1016/s0022-5320(84)80024-6. View

17.

Carlemalm E, Villiger W, Hobot J, Acetarin J, Kellenberger E . Low temperature embedding with Lowicryl resins: two new formulations and some applications. J Microsc. 1985; 140(Pt 1):55-63. DOI: 10.1111/j.1365-2818.1985.tb02660.x. View

18.

Locker R, Wild D . A comparative study of high molecular weight proteins in various types of muscle across the animal kingdom. J Biochem. 1986; 99(5):1473-84. DOI: 10.1093/oxfordjournals.jbchem.a135617. View

19.

Hu D, Kimura S, Maruyama K . Sodium dodecyl sulfate gel electrophoresis studies of connectin-like high molecular weight proteins of various types of vertebrate and invertebrate muscles. J Biochem. 1986; 99(5):1485-92. DOI: 10.1093/oxfordjournals.jbchem.a135618. View

20.

Maruyama K . Connectin, an elastic filamentous protein of striated muscle. Int Rev Cytol. 1986; 104:81-114. DOI: 10.1016/s0074-7696(08)61924-5. View