Expression of NPAT, a Novel Substrate of Cyclin E-CDK2, Promotes S-phase Entry

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 1998 Mar 21

PMID 9472014

Citations 81

Authors

J Zhao

B Dynlacht

T Imai

T Hori

E Harlow

Affiliations

Soon will be listed here.

Abstract

To understand the mechanisms by which CDKs regulate cell cycle progression, it is necessary to identify and characterize the physiological substrates of these kinases. We have developed a screening method to identify novel CDK substrates. One of the cDNAs identified in the screen is identical to the recently isolated NPAT gene. Here we show that NPAT associates with cyclin E-CDK2 in vivo and can be phosphorylated by this CDK. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry, and this effect is enhanced by coexpression of cyclin E-CDK2. These results suggest that NPAT is a substrate of cyclin E-CDK2 and plays a role in S-phase entry.

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