Dynamics of the Murine Humoral Immune Response to Neisseria Meningitis Group B Capsular Polysaccharide

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1998 Feb 7

PMID 9453603

Citations 3

Authors

J Colino

I Outschoorn

Affiliations

Soon will be listed here.

Abstract

Immunization with Neisseria meningitidis group B capsular polysaccharide (CpsB) elicited responses in adult mice that showed the typical dynamic characteristics of the response to a thymus-independent antigen, in contrast to the thymus-dependent behavior of antibody responses to CpsC. The former had a short latent period and showed a rapid increase in serum antibodies that peaked at day 5, and immunoglobulin M (IgM) was the major isotype even though IgG (mainly IgG2a and IgG2b) was also detectable. This response was of short duration, and the specific antibodies were rapidly cleared from the circulation. The secondary responses were similar in magnitude, kinetics, IgM predominance, and IgG distribution. Nevertheless, a threefold IgG increase, a correlation between IgM and IgG levels, and dose-dependent secondary responses were observed. Hyperimmunization considerably reinforced these responses: 10-fold for IgM and 300-fold for IgG. This favored isotype switch was accompanied by a progressive change in the subclass distribution to IgG3 (62%) and IgG1 (28%), along with the possible generation of B-cell memory. The results indicate that CpsB is being strictly thymus independent and suggest that unresponsiveness to purified CpsB is due to tolerance.

Citing Articles

Gut Microbiota Contributes to Resistance Against Pneumococcal Pneumonia in Immunodeficient Rag Mice.

Felix K, Jaimez I, Nguyen T, Ma H, Raslan W, Klinger C Front Cell Infect Microbiol. 2018; 8:118.

PMID: 29755958 PMC: 5932343. DOI: 10.3389/fcimb.2018.00118.

The nature of an in vivo anti-capsular polysaccharide response is markedly influenced by the composition and/or architecture of the bacterial subcapsular domain.

Arjunaraja S, Massari P, Wetzler L, Lees A, Colino J, Snapper C J Immunol. 2011; 188(2):569-77.

PMID: 22156342 PMC: 3253264. DOI: 10.4049/jimmunol.1101446.

Vaccination effect of interleukin-6-producing pancreatic cancer cells in nude mice: a model of tumor prevention and treatment in immune-compromised patients.

Yano T, Ishikura H, Ogawa Y, Kondo S, Kato H, Yoshiki T Jpn J Cancer Res. 2001; 92(1):83-7.

PMID: 11173548 PMC: 5926582. DOI: 10.1111/j.1349-7006.2001.tb01051.x.

References

Tsai C . The analysis of lipopolysaccharide (endotoxin) in meningococcal polysaccharide vaccines by silver staining following SDS-polyacrylamide gel electrophoresis. J Biol Stand. 1986; 14(1):25-33. DOI: 10.1016/s0092-1157(86)80006-3. View

Svennerholm L . Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta. 1957; 24(3):604-11. DOI: 10.1016/0006-3002(57)90254-8. View

Finne J, Bitter-Suermann D, Goridis C, Finne U . An IgG monoclonal antibody to group B meningococci cross-reacts with developmentally regulated polysialic acid units of glycoproteins in neural and extraneural tissues. J Immunol. 1987; 138(12):4402-7. View

Holton 3rd O, Black C, Parker R, Covell D, Barbet J, Sieber S . Biodistribution of monoclonal IgG1, F(ab')2, and Fab' in mice after intravenous injection. Comparison between anti-B cell (anti-Lyb8.2) and irrelevant (MOPC-21) antibodies. J Immunol. 1987; 139(9):3041-9. View

Zhang J, Liu Y, MacLennan I, Gray D, Lane P . B cell memory to thymus-independent antigens type 1 and type 2: the role of lipopolysaccharide in B memory induction. Eur J Immunol. 1988; 18(9):1417-24. DOI: 10.1002/eji.1830180918. View

Lifely M, Wang Z . Immune responses in mice to different noncovalent complexes of meningococcal B polysaccharide and outer membrane proteins. Infect Immun. 1988; 56(12):3221-7. PMC: 259728. DOI: 10.1128/iai.56.12.3221-3227.1988. View

Czerkinsky C, Moldoveanu Z, Mestecky J, Nilsson L, OUCHTERLONY O . A novel two colour ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells. J Immunol Methods. 1988; 115(1):31-7. DOI: 10.1016/0022-1759(88)90306-7. View

Rubinstein L, STEIN K . Murine immune response to the Neisseria meningitidis group C capsular polysaccharide. I. Ontogeny. J Immunol. 1988; 141(12):4352-6. View

Taylor C, Bright R . T-cell modulation of the antibody response to bacterial polysaccharide antigens. Infect Immun. 1989; 57(1):180-5. PMC: 313065. DOI: 10.1128/iai.57.1.180-185.1989. View

10.

Jennings H, Gamian A, Michon F, Ashton F . Unique intermolecular bactericidal epitope involving the homosialopolysaccharide capsule on the cell surface of group B Neisseria meningitidis and Escherichia coli K1. J Immunol. 1989; 142(10):3585-91. View

11.

Greis C, Rosner H . c-pathway polysialogangliosides in the nervous tissue of vertebrates, reacting with the monoclonal antibody Q211. Brain Res. 1990; 517(1-2):105-10. DOI: 10.1016/0006-8993(90)91014-8. View

12.

Donnelly J, Deck R, Liu M . Immunogenicity of a Haemophilus influenzae polysaccharide-Neisseria meningitidis outer membrane protein complex conjugate vaccine. J Immunol. 1990; 145(9):3071-9. View

13.

Davies R, Wall R, Borriello S . Comparison of methods for the analysis of outer membrane antigens of Neisseria meningitidis by western blotting. J Immunol Methods. 1990; 134(2):215-25. DOI: 10.1016/0022-1759(90)90383-7. View

14.

Raff H, Bradley C, Brady W, Donaldson K, Lipsich L, Maloney G . Comparison of functional activities between IgG1 and IgM class-switched human monoclonal antibodies reactive with group B streptococci or Escherichia coli K1. J Infect Dis. 1991; 163(2):346-54. DOI: 10.1093/infdis/163.2.346. View

15.

Lifely M, Roberts S, Shepherd W, Esdaile J, Wang Z, Cleverly A . Immunogenicity in adult males of a Neisseria meningitidis group B vaccine composed of polysaccharide complexed with outer membrane proteins. Vaccine. 1991; 9(1):60-6. DOI: 10.1016/0264-410x(91)90318-z. View

16.

Devi S, Robbins J, Schneerson R . Antibodies to poly[(2----8)-alpha-N-acetylneuraminic acid] and poly[(2----9)-alpha-N-acetylneuraminic acid] are elicited by immunization of mice with Escherichia coli K92 conjugates: potential vaccines for groups B and C meningococci and E. coli K1. Proc Natl Acad Sci U S A. 1991; 88(16):7175-9. PMC: 52256. DOI: 10.1073/pnas.88.16.7175. View

17.

Plikaytis B, Turner S, Gheesling L, Carlone G . Comparisons of standard curve-fitting methods to quantitate Neisseria meningitidis group A polysaccharide antibody levels by enzyme-linked immunosorbent assay. J Clin Microbiol. 1991; 29(7):1439-46. PMC: 270131. DOI: 10.1128/jcm.29.7.1439-1446.1991. View

18.

Liu M, Friedman A, Oliff A, Tai J, Martinez D, Deck R . A vaccine carrier derived from Neisseria meningitidis with mitogenic activity for lymphocytes. Proc Natl Acad Sci U S A. 1992; 89(10):4633-7. PMC: 49137. DOI: 10.1073/pnas.89.10.4633. View

19.

Jennings H . Further approaches for optimizing polysaccharide-protein conjugate vaccines for prevention of invasive bacterial disease. J Infect Dis. 1992; 165 Suppl 1:S156-9. DOI: 10.1093/infdis/165-supplement_1-s156. View

20.

Baker P . T cell regulation of the antibody response to bacterial polysaccharide antigens: an examination of some general characteristics and their implications. J Infect Dis. 1992; 165 Suppl 1:S44-8. DOI: 10.1093/infdis/165-supplement_1-s44. View