A Novel Two Colour ELISPOT Assay. I. Simultaneous Detection of Distinct Types of Antibody-secreting Cells

Overview

Journal J Immunol Methods

Publisher Elsevier

Specialties Allergy & Immunology
Pathology

Date 1988 Nov 25

PMID 3057075

Citations 43

Authors

C Czerkinsky

Z Moldoveanu

J Mestecky

L A Nilsson

O OUCHTERLONY

Affiliations

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Abstract

A novel assay system has been developed which is based on the ELISPOT methodology and employs a combination of two immunoenzyme visualization systems yielding distinct colour products. This variation permits the simultaneous enumeration of two different types of cell secreting antigenically distinct products. Optimal conditions for the concurrent detection of human mononuclear cells secreting IgG or IgA antibodies are described.

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