POU Transcription Factors Brn-3a and Brn-3b Interact with the Estrogen Receptor and Differentially Regulate Transcriptional Activity Via an Estrogen Response Element

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1998 Feb 3

PMID 9448000

Citations 15

Authors

V Budhram-Mahadeo

M Parker

D S Latchman

Affiliations

Soon will be listed here.

Abstract

The estrogen receptor (ER) modulates transcription by forming complexes with other proteins and then binding to the estrogen response element (ERE). We have identified a novel interaction of this receptor with the POU transcription factors Brn-3a and Brn-3b which was independent of ligand binding. By pull-down assays and the yeast two-hybrid system, the POU domain of Brn-3a and Brn-3b was shown to interact with the DNA-binding domain of the ER. Brn-3-ER interactions also affect transcriptional activity of an ERE-containing promoter, such that in estradiol-stimulated cells, Brn-3b strongly activated the promoter via the ERE, while Brn-3a had a mild inhibitory effect. The POU domain of Brn-3b which interacts with the ER was sufficient to confer this activation potential, and the change of a single amino acid in the first helix of the POU homeodomain of Brn-3a to its equivalent in Brn-3b can change the mild repressive effect of Brn-3a to a stimulatory Brn-3b-like effect. These observations and their implications for transcriptional regulation by the ER are discussed.

Citing Articles

Linking metabolic dysfunction with cardiovascular diseases: Brn-3b/POU4F2 transcription factor in cardiometabolic tissues in health and disease.

Budhram-Mahadeo V, Solomons M, Mahadeo-Heads E Cell Death Dis. 2021; 12(3):267.

PMID: 33712567 PMC: 7955040. DOI: 10.1038/s41419-021-03551-9.

Transcriptional regulation of prolactin in a euryhaline teleost: Characterisation of gene promoters through in silico and transcriptome analyses.

Seale A, Malintha G, Celino-Brady F, Head T, Belcaid M, Yamaguchi Y J Neuroendocrinol. 2020; 32(11):e12905.

PMID: 32996203 PMC: 8612711. DOI: 10.1111/jne.12905.

Transcription factors Brn-3α and TRIM16 in cancers, association with hormone reception.

Spirina L, Yunusova N, Kondakova I, Tarasenko N Heliyon. 2019; 5(8):e02090.

PMID: 31463379 PMC: 6708992. DOI: 10.1016/j.heliyon.2019.e02090.

ERα is required for suppressing OCT4-induced proliferation of breast cancer cells via DNMT1/ISL1/ERK axis.

Jin X, Li Y, Guo Y, Jia Y, Qu H, Lu Y Cell Prolif. 2019; 52(4):e12612.

PMID: 31012189 PMC: 6668970. DOI: 10.1111/cpr.12612.

Hsp-27 induction requires POU4F2/Brn-3b TF in doxorubicin-treated breast cancer cells, whereas phosphorylation alters its cellular localisation following drug treatment.

Fujita R, Ounzain S, Wang A, Heads R, Budhram-Mahadeo V Cell Stress Chaperones. 2011; 16(4):427-39.

PMID: 21279488 PMC: 3118820. DOI: 10.1007/s12192-011-0256-8.

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