New Core Promoter Element in RNA Polymerase II-dependent Transcription: Sequence-specific DNA Binding by Transcription Factor IIB

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 1998 Feb 21

PMID 9420329

Citations 163

Authors

T LaGrange

A N Kapanidis

H Tang

D Reinberg

R H Ebright

Affiliations

Soon will be listed here.

Abstract

A sequence element located immediately upstream of the TATA element, and having the consensus sequence 5'-G/C-G/C-G/A-C-G-C-C-3', affects the ability of transcription factor IIB to enter transcription complexes and support transcription initiation. The sequence element is recognized directly by the transcription factor IIB. Recognition involves alpha-helices 4' and 5' of IIB, which comprise a helix-turn-helix DNA-binding motif. These observations establish that transcription initiation involves a fourth core promoter element, the IIB recognition element (BRE), in addition to the TATA element, the initiator element, and the downstream promoter element, and involves a second sequence-specific general transcription factor, IIB, in addition to transcription factor IID.

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