Receptor-mediated Protein Kinase Activation and the Mechanism of Transmembrane Signaling in Bacterial Chemotaxis

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1998 Feb 21

PMID 9405352

Citations 42

Authors

Y Liu

M LEVIT

R Lurz

M G Surette

J B Stock

Affiliations

Soon will be listed here.

Abstract

Chemotaxis responses of Escherichia coli and Salmonella are mediated by type I membrane receptors with N-terminal extracytoplasmic sensing domains connected by transmembrane helices to C-terminal signaling domains in the cytoplasm. Receptor signaling involves regulation of an associated protein kinase, CheA. Here we show that kinase activation by a soluble signaling domain construct involves the formation of a large complex, with approximately 14 receptor signaling domains per CheA dimer. Electron microscopic examination of these active complexes indicates a well defined bundle composed of numerous receptor filaments. Our findings suggest a mechanism for transmembrane signaling whereby stimulus-induced changes in lateral packing interactions within an array of receptor-sensing domains at the cell surface perturb an equilibrium between active and inactive receptor-kinase complexes within the cytoplasm.

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