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Detection of Immunoglobulin G (IgG) and IgM Antibodies to Toxoplasma Gondii: Evaluation of Four Commercial Immunoassay Systems

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1997 Dec 17
PMID 9399544
Citations 20
Authors
W T Hofgartner
S R Swanzy
R M Bacina
J Condon
M Gupta
P E Matlock
D L Bergeron
J J PLORDE
T R Fritsche
Affiliations
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Abstract

A comparative evaluation of the following commercial immunoassays for the determination of antibodies to Toxoplasma gondii was performed: Behring Diagnostics OPUS Toxo G and Toxo M, Abbott Diagnostics IMX Toxo-IgG 2.0 and Toxo-IgM, Sanofi Diagnostics Pasteur Platelia Toxo IgG and Toxo IgM, and bioMérieux Vitek VIDAS Toxo IgG and IgM. Of 676 specimens that were tested for Toxoplasma-specific immunoglobulin G (IgG) antibodies, 26% were reactive by all methods while 8% displayed some discrepancy. Of 718 specimens that were tested for Toxoplasma-specific IgM antibodies, 3% were reactive by all methods while 10% displayed some discrepancy. Analysis of discrepant specimens revealed performance shortcomings with all IgM-specific assays. The impact of such shortcomings is magnified in a population with a low prevalence of toxoplasmosis.

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