Both Pituitary and Placental Growth Hormone Transcripts Are Expressed in Human Peripheral Blood Mononuclear Cells (PBMC)

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1997 Nov 21

PMID 9367422

Citations 2

Authors

L Melen

G Hennen

R P Dullaart

E Heinen

A Igout

Affiliations

Soon will be listed here.

Abstract

The hGH-V gene codes for a variant of human pituitary growth hormone (hGH-N) named placental growth hormone (hPGH). hPGH shares 93% amino acid identity with hGH-N. Until now the hGH-V gene was considered to be exclusively expressed in human placenta, where it replaces maternal circulating hGH-N at the end of pregnancy. In this study we investigated by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis hGH-N, and hGH-V, gene expression in PBMC in men, women and pregnant women. We have demonstrated that hGH-N and hGH-V transcripts are simultaneously produced by PBMC in both men and women as well as pregnant women. The PBMC of a PIT-1-negative woman expressed only the hGH-V transcript, but not the hGH-N one as expected. In conclusion, hGH-V mRNA is expressed by cells other than the syncytiotrophoblast, is not regulated by PIT-1, and may be involved in immune regulation, as is pituitary GH.

Citing Articles

Non-pituitary GH regulation of the tissue microenvironment.

Chesnokova V, Melmed S Endocr Relat Cancer. 2023; 30(7).

PMID: 37066857 PMC: 10247477. DOI: 10.1530/ERC-23-0028.

Placental growth hormones.

Lacroix M, Guibourdenche J, Frendo J, Pidoux G, Evain-Brion D Endocrine. 2003; 19(1):73-9.

PMID: 12583604 DOI: 10.1385/ENDO:19:1:73.

References

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Cattini P, Eberhardt N . Regulated expression of chimaeric genes containing the 5'-flanking regions of human growth hormone-related genes in transiently transfected rat anterior pituitary tumor cells. Nucleic Acids Res. 1987; 15(3):1297-309. PMC: 340525. DOI: 10.1093/nar/15.3.1297. View

Barsh G, Seeburg P, Gelinas R . The human growth hormone gene family: structure and evolution of the chromosomal locus. Nucleic Acids Res. 1983; 11(12):3939-58. PMC: 326017. DOI: 10.1093/nar/11.12.3939. View

Hennen G, Frankenne F, Pirens G, Gomez F, Closset J, Schaus C . New chorionic GH-like antigen revealed by monoclonal antibody radioimmunoassays. Lancet. 1985; 1(8425):399. DOI: 10.1016/s0140-6736(85)91420-5. View

Hennen G, Frankenne F, Closset J, Gomez F, Smal J, el Khayat N . [Physiology of the growth hormone in pregnancy and discovery of a human placental growth hormone]. Bull Mem Acad R Med Belg. 1986; 141(1):111-9. View

Hirt H, Kimelman J, Birnbaum M, Chen E, Seeburg P, Eberhardt N . The human growth hormone gene locus: structure, evolution, and allelic variations. DNA. 1987; 6(1):59-70. DOI: 10.1089/dna.1987.6.59. View

Weigent D, Baxter J, Wear W, Smith L, Bost K, Blalock J . Production of immunoreactive growth hormone by mononuclear leukocytes. FASEB J. 1988; 2(12):2812-8. DOI: 10.1096/fasebj.2.12.3044906. View

Bodner M, Castrillo J, Theill L, Deerinck T, Ellisman M, Karin M . The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein. Cell. 1988; 55(3):505-18. DOI: 10.1016/0092-8674(88)90037-2. View

Ingraham H, Chen R, Mangalam H, Elsholtz H, FLYNN S, Lin C . A tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotype. Cell. 1988; 55(3):519-29. DOI: 10.1016/0092-8674(88)90038-4. View

10.

Kelley K . Growth hormone, lymphocytes and macrophages. Biochem Pharmacol. 1989; 38(5):705-13. DOI: 10.1016/0006-2952(89)90222-0. View

11.

Chen E, Liao Y, Smith D, Barrera-Saldana H, Gelinas R, Seeburg P . The human growth hormone locus: nucleotide sequence, biology, and evolution. Genomics. 1989; 4(4):479-97. DOI: 10.1016/0888-7543(89)90271-1. View

12.

Frankenne F, Scippo M, Van Beeumen J, Igout A, Hennen G . Identification of placental human growth hormone as the growth hormone-V gene expression product. J Clin Endocrinol Metab. 1990; 71(1):15-8. DOI: 10.1210/jcem-71-1-15. View

13.

Pfaffle R, DiMattia G, Parks J, Brown M, Wit J, Jansen M . Mutation of the POU-specific domain of Pit-1 and hypopituitarism without pituitary hypoplasia. Science. 1992; 257(5073):1118-21. DOI: 10.1126/science.257.5073.1118. View

14.

Delhase M, Vergani P, Malur A, Hooghe-Peters E, Hooghe R . The transcription factor Pit-1/GHF-1 is expressed in hemopoietic and lymphoid tissues. Eur J Immunol. 1993; 23(4):951-5. DOI: 10.1002/eji.1830230428. View

15.

Nachtigal M, Nickel B, Cattini P . Pituitary-specific repression of placental members of the human growth hormone gene family. A possible mechanism for locus regulation. J Biol Chem. 1993; 268(12):8473-9. View

16.

Scippo M, Frankenne F, Hooghe-Peters E, Igout A, Velkeniers B, Hennen G . Syncytiotrophoblastic localization of the human growth hormone variant mRNA in the placenta. Mol Cell Endocrinol. 1993; 92(2):R7-13. DOI: 10.1016/0303-7207(93)90019-g. View

17.

Bradley W, Emmanuel P, GOOD R, Day N . Detection of cell specific cluster determinant expression by reverse transcriptase polymerase chain reaction. J Immunol Methods. 1994; 169(1):73-82. DOI: 10.1016/0022-1759(94)90126-0. View

18.

Maggiano N, Piantelli M, Ricci R, Larocca L, Capelli A, Ranelletti F . Detection of growth hormone-producing cells in human thymus by immunohistochemistry and non-radioactive in situ hybridization. J Histochem Cytochem. 1994; 42(10):1349-54. DOI: 10.1177/42.10.7930517. View

19.

Bamberger A, Bamberger C, Pu L, Puy L, Loh Y, Asa S . Expression of pit-1 messenger ribonucleic acid and protein in the human placenta. J Clin Endocrinol Metab. 1995; 80(7):2021-6. DOI: 10.1210/jcem.80.7.7608249. View

20.

Palmetshofer A, Zechner D, Luger T, Barta A . Splicing variants of the human growth hormone mRNA: detection in pituitary, mononuclear cells and dermal fibroblasts. Mol Cell Endocrinol. 1995; 113(2):225-34. DOI: 10.1016/0303-7207(95)03633-i. View