Effects of Epidermal Growth Factor and Insulin on Migration and Proliferation of Primary Cultured Rabbit Gastric Epithelial Cells

Overview

Journal J Gastroenterol

Specialty Gastroenterology

Date 1997 Nov 14

PMID 9349980

Citations 3

Authors

K Maehiro

S Watanabe

M Hirose

R Iwazaki

H Miwa

N Sato

Affiliations

Soon will be listed here.

Abstract

We assessed the influence of epidermal growth factor (EGF) and insulin on gastric epithelial restoration in vitro. Rabbit gastric epithelial cells were cultured and formed a complete monolayer cell sheet in 2 days. We created a wound (1.8 +/- 0.05 mm2) by denuding an area of cells, and EGF (0.1-30 ng/ml) and/or insulin (1 nM-1 microM) was added. The restoration process, which included cell migration and proliferation, was monitored by measuring the cell-free area every 12 h for 2 days. Proliferating cells were detected by sequential staining with bromodeoxyuridine (BrdU). Control cells showed complete repair in 36-48 h and restoration was accelerated dose-dependently by EGF or insulin. EGF plus insulin further accelerated restoration, which was then completed in 12-24 h. EGF and/or insulin increased the number of BrdU- positive cells. The results indicated that EGF and insulin additively accelerated gastric epithelial wound repair by stimulating both the migration and the proliferation of gastric epithelial cells (particularly the former).

Citing Articles

Effects of epidermal growth factor on the growth of human gastric cancer cell and the implanted tumor of nude mice.

Xia L, Yuan Y, Xu C, Zhang Y, Qiao M World J Gastroenterol. 2002; 8(3):455-8.

PMID: 12046069 PMC: 4656420. DOI: 10.3748/wjg.v8.i3.455.

Migration of primary cultured rabbit gastric epithelial cells requires intact protein kinase C and Ca2+/calmodulin activity.

Ranta-Knuuttila T, Kiviluoto T, Mustonen H, Puolakkainen P, Watanabe S, Sato N Dig Dis Sci. 2002; 47(5):1008-14.

PMID: 12018895 DOI: 10.1023/a:1015025704589.

Ammonia, hydrogen peroxide, and monochloramine retard gastric epithelial restoration in rabbit cultured cell model.

Sato K, Watanabe S, Yoshizawa T, Hirose M, Murai T, Sato N Dig Dis Sci. 2000; 44(12):2429-34.

PMID: 10630492 DOI: 10.1023/a:1026670518567.

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