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Effects of Epidermal Growth Factor on the Growth of Human Gastric Cancer Cell and the Implanted Tumor of Nude Mice

Overview

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2002 Jun 5

PMID 12046069

Citations 7

Authors

Authors

Lu Xia

Yao-Zong Yuan

Chun-Di Xu

Yong-Pin Zhang

Ming-Ming Qiao

Affiliations

Affiliations

Soon will be listed here.

Abstract

Aim: Epidermal growth factor (EGF) plays an important role in the regulation of gastrointestinal tissue growth and development, and it can stimulate epithelial proliferation, cell differentiation and growth. It has been established that the EGF can promote gastric cytoprotection and ulcer healing. But the potential ability of EGF to regulate the gastric cancer growth is unknown. This study is to investigate the influence of EGF on human gastric cancer cell and the implanted tumor growth of nude mice.

Methods: The cell growth rates of human gastric adenocarcinoma cell lines MKN-28, MKN-45, SGC-7901 and normal human gastric epithelial cells 3T3 were assessed when incubated with recombinant human EGF (rhEGF, 0.05, 0.1, 0.5, 1.0, 10, 50, 100 mg.L(-1)) using MTT method. The cells of MKN-28, MKN-45, SGC-7901 (gastric cancer tissue 1.5mm(3)) were implanted in the BALB/cA nude mice for 10 days. The EGF was given intraperitoneally (15, 30, 60 microg.kg(-1)) for 3 weeks. The body weights of the tumor-bearing animals and their tumor mass were measured afterwards to assess the mitogenic effect of rhEGF in the nude mice.

Results: Within the concentration range of 0.05-100mg.L(-1), rhEGF could increase the cell growth of normal 3T3 cells (cell growth rate 100% vs 102.8%, P<0.05), but partially restrain the gastric cancer cell growth. The latter effect was related to cell differentiation. In 15-60 microg/kg rhEGF groups, the mean implanted tumor mass of MKN-28 cell were 1.75 g, 1.91 g, 2.08 g/NS group 1.97 g (P>0.05), the mean tumor mass of SGC-7901 cell were 1.53 g, 1.07 g, 1.20 g/NS group 1.07 g (P>0.05), and for MKN-45 cell, the tumor mass were respectively 1.92 g, 1.29 g, 1.77 /NS group 1.82 g (P>0.05). So rhEGF had no obvious effect on implanted MKN-28, SGC-7901 and MKN-45 tumor growth.

Conclusion: EGF has no stimulating effect on the human gastric cancer cell growth neither in vitro nor in vivo.

Citing Articles

Epidermal growth factor in clinical practice - a review of its biological actions, clinical indications and safety implications.

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Recombinant human epidermal growth factor treatment of radiation-induced severe oral mucositis in patients with head and neck malignancies.

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Expression of tumor related genes NGX6, NAG-7, BRD7 in gastric and colorectal cancer.

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Expression of NGF family and their receptors in gastric carcinoma: a cDNA microarray study.

Du J, Dou K, Peng S, Qian B, Xiao H, Liu F World J Gastroenterol. 2003; 9(7):1431-4.

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References

1.

Ross J, McKenna B . The HER-2/neu oncogene in tumors of the gastrointestinal tract. Cancer Invest. 2001; 19(5):554-68. DOI: 10.1081/cnv-100103852. View

2.

Becker K, Keller G, Hoefler H . The use of molecular biology in diagnosis and prognosis of gastric cancer. Surg Oncol. 2001; 9(1):5-11. DOI: 10.1016/s0960-7404(00)00016-5. View

3.

Konturek J, Hengst K, Konturek S, Domschke W . Epidermal growth factor in gastric ulcer healing by nocloprost, a stable prostaglandin E2 derivative. Scand J Gastroenterol. 1997; 32(10):980-4. DOI: 10.3109/00365529709011213. View

4.

Arakawa T, Watanabe T, Fukuda T, Higuchi K, Takaishi O, Yamasaki K . Indomethacin treatment during initial period of acetic acid-induced rat gastric ulcer healing promotes persistent polymorphonuclear cell-infiltration and increases future ulcer recurrence. Possible mediation of prostaglandins. Dig Dis Sci. 1996; 41(10):2055-61. DOI: 10.1007/BF02093610. View

5.

Koyama S, Maruyama T, Adachi S . Expression of epidermal growth factor receptor and CD44 splicing variants sharing exons 6 and 9 on gastric and esophageal carcinomas: a two-color flow-cytometric analysis. J Cancer Res Clin Oncol. 1999; 125(1):47-54. DOI: 10.1007/s004320050241. View

6.

Ma L, Liu E, Chow J, Wang J, Cho C . Interactions of EGF and ornithine decarboxylase activity in the regulation of gastric mucus synthesis in cigarette smoke exposed rats. Chin J Physiol. 2000; 42(3):137-43. View

7.

Zarrilli R, Ricci V, Romano M . Molecular response of gastric epithelial cells to Helicobacter pylori-induced cell damage. Cell Microbiol. 2001; 1(2):93-9. DOI: 10.1046/j.1462-5822.1999.00018.x. View

8.

Konturek P, Ernst H, Brzozowski T, Ihlm A, Hahn E, Konturek S . Expression of epidermal growth factor and transforming growth factor-alpha after exposure of rat gastric mucosa to stress. Scand J Gastroenterol. 1996; 31(3):209-16. DOI: 10.3109/00365529609004868. View

9.

Romano M, Ricci V, Di Popolo A, Sommi P, Del Vecchio Blanco C, Bruni C . Helicobacter pylori upregulates expression of epidermal growth factor-related peptides, but inhibits their proliferative effect in MKN 28 gastric mucosal cells. J Clin Invest. 1998; 101(8):1604-13. PMC: 508741. DOI: 10.1172/JCI1174. View

10.

Maehiro K, Watanabe S, Hirose M, Iwazaki R, Miwa H, Sato N . Effects of epidermal growth factor and insulin on migration and proliferation of primary cultured rabbit gastric epithelial cells. J Gastroenterol. 1997; 32(5):573-8. DOI: 10.1007/BF02934104. View

11.

Murayama Y, Miyagawa J, Higashiyama S, Kondo S, Yabu M, Isozaki K . Localization of heparin-binding epidermal growth factor-like growth factor in human gastric mucosa. Gastroenterology. 1995; 109(4):1051-9. DOI: 10.1016/0016-5085(95)90562-6. View

12.

Sanz-Ortega J, Steinberg S, Moro E, Saez M, Lopez J, Sierra E . Comparative study of tumor angiogenesis and immunohistochemistry for p53, c-ErbB2, c-myc and EGFr as prognostic factors in gastric cancer. Histol Histopathol. 2000; 15(2):455-62. DOI: 10.14670/HH-15.455. View

13.

Miyazaki Y, Hiraoka S, Tsutsui S, Kitamura S, Shinomura Y, Matsuzawa Y . Epidermal growth factor receptor mediates stress-induced expression of its ligands in rat gastric epithelial cells. Gastroenterology. 2001; 120(1):108-16. DOI: 10.1053/gast.2001.20950. View

14.

Garcia I, Vizoso F, Andicoechea A, Raigoso P, VEREZ P, Alexandre E . Clinical significance of epidermal growth factor receptor content in gastric cancer. Int J Biol Markers. 2001; 16(3):183-8. DOI: 10.1177/172460080101600305. View

15.

Messa C, Russo F, Pricci M, Di Leo A . Epidermal growth factor and 17beta-estradiol effects on proliferation of a human gastric cancer cell line (AGS). Scand J Gastroenterol. 2000; 35(7):753-8. DOI: 10.1080/003655200750023444. View

16.

Choi J, Kim H, Lim H, Nam D, Kim H, Yi S . Detection of transforming growth factor-alpha in the serum of gastric carcinoma patients. Oncology. 1999; 57(3):236-41. DOI: 10.1159/000012037. View

17.

Luan F, Wang M, You W . [The correlation of TGF-alpha, EGFR in precancerous lesions and carcinoma of stomach with PCNA expression]. Zhonghua Bing Li Xue Za Zhi. 1997; 26(1):31-4. View

18.

Granelli P, Fichera G, Zennaro F, Siardi C, De Ruberto F, Fregoni F . Expression of the epidermal growth factor receptor gene in human intestinal metaplasia: a preliminary report. Scand J Gastroenterol. 1997; 32(5):485-9. DOI: 10.3109/00365529709025086. View

19.

Wagner S, Beil W, Westermann J, Logan R, Bock C, Trautwein C . Regulation of gastric epithelial cell growth by Helicobacter pylori: offdence for a major role of apoptosis. Gastroenterology. 1997; 113(6):1836-47. DOI: 10.1016/s0016-5085(97)70003-9. View

20.

Uribe J, Barrett K . Nonmitogenic actions of growth factors: an integrated view of their role in intestinal physiology and pathophysiology. Gastroenterology. 1997; 112(1):255-68. View