The Glucose Transport System of the Hyperthermophilic Anaerobic Bacterium Thermotoga Neapolitana

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1996 Aug 1

PMID 9285772

Citations 12

Authors

Galperin

Noll

Romano

Affiliations

Soon will be listed here.

Abstract

The glucose transport system of the extremely thermophilic anaerobic bacterium Thermotoga neapolitana was studied with the nonmetabolizable glucose analog 2-deoxy-D-glucose (2-DOG). T. neapolitana accumulated 2-DOG against a concentration gradient in an intracellular free sugar pool that was exchangeable with external source of energy, such as pyruvate, and was inhibited by arsenate and gramicidin D. There was no phosphoenolpyruvate-dependent phosphorylation of glucose, 2-DOG, or fructose by cell extracts or toluene-treated cells, indicating the absence of a phosphoenolpyruvate:sugar phosphotransferase system. These data indicate that D-glucose is taken up by T. neapolitana via an active transport system that is energized by an ion gradient generated by ATP, derived from substrate-level phosphorylation.

Citing Articles

Effect of Cultivation Parameters on Fermentation and Hydrogen Production in the Phylum .

Lanzilli M, Esercizio N, Vastano M, Xu Z, Nuzzo G, Gallo C Int J Mol Sci. 2021; 22(1).

PMID: 33396970 PMC: 7795431. DOI: 10.3390/ijms22010341.

Identification of the ATPase Subunit of the Primary Maltose Transporter in the Hyperthermophilic Anaerobe Thermotoga maritima.

Singh R, White D, Blum P Appl Environ Microbiol. 2017; 83(18).

PMID: 28687653 PMC: 5583491. DOI: 10.1128/AEM.00930-17.

Contribution of Pentose Catabolism to Molecular Hydrogen Formation by Targeted Disruption of Arabinose Isomerase (araA) in the Hyperthermophilic Bacterium Thermotoga maritima.

White D, Singh R, Rudrappa D, Mateo J, Kramer L, Freese L Appl Environ Microbiol. 2016; 83(4).

PMID: 27940539 PMC: 5288831. DOI: 10.1128/AEM.02631-16.

Hyperthermophilic Thermotoga species differ with respect to specific carbohydrate transporters and glycoside hydrolases.

Frock A, Gray S, Kelly R Appl Environ Microbiol. 2012; 78(6):1978-86.

PMID: 22247137 PMC: 3298158. DOI: 10.1128/AEM.07069-11.

Physiological characteristics of the extreme thermophile Caldicellulosiruptor saccharolyticus: an efficient hydrogen cell factory.

Willquist K, Zeidan A, van Niel E Microb Cell Fact. 2010; 9:89.

PMID: 21092203 PMC: 3003633. DOI: 10.1186/1475-2859-9-89.

References

Cook G, Janssen P, Morgan H . Uncoupler-Resistant Glucose Uptake by the Thermophilic Glycolytic Anaerobe Thermoanaerobacter thermosulfuricus (Clostridium thermohydrosulfuricum). Appl Environ Microbiol. 1993; 59(9):2984-90. PMC: 182396. DOI: 10.1128/aem.59.9.2984-2990.1993. View

Kengen S, de Bok F, van Loo N, Dijkema C, Stams A, de Vos W . Evidence for the operation of a novel Embden-Meyerhof pathway that involves ADP-dependent kinases during sugar fermentation by Pyrococcus furiosus. J Biol Chem. 1994; 269(26):17537-41. View

Kornberg H, Reeves R . Inducible phosphoenolpyruvate-dependent hexose phosphotransferase activities in Escherichia coli. Biochem J. 1972; 128(5):1339-44. PMC: 1174022. DOI: 10.1042/bj1281339. View

Adams M . Biochemical diversity among sulfur-dependent, hyperthermophilic microorganisms. FEMS Microbiol Rev. 1994; 15(2-3):261-77. DOI: 10.1111/j.1574-6976.1994.tb00139.x. View

Gupta R, Stetter K, Woese C . Were the original eubacteria thermophiles?. Syst Appl Microbiol. 1987; 9:34-9. DOI: 10.1016/s0723-2020(87)80053-x. View

Kashket E . Proton motive force in growing Streptococcus lactis and Staphylococcus aureus cells under aerobic and anaerobic conditions. J Bacteriol. 1981; 146(1):369-76. PMC: 217092. DOI: 10.1128/jb.146.1.369-376.1981. View

Woese C . Bacterial evolution. Microbiol Rev. 1987; 51(2):221-71. PMC: 373105. DOI: 10.1128/mr.51.2.221-271.1987. View

Gilles A, Glaser P, Perrier V, Meier A, Longin R, Sebald M . Zinc, a structural component of adenylate kinases from gram-positive bacteria. J Bacteriol. 1994; 176(2):520-3. PMC: 205078. DOI: 10.1128/jb.176.2.520-523.1994. View

Childers S, Vargas M, Noll K . Improved Methods for Cultivation of the Extremely Thermophilic Bacterium Thermotoga neapolitana. Appl Environ Microbiol. 1992; 58(12):3949-53. PMC: 183209. DOI: 10.1128/aem.58.12.3949-3953.1992. View

10.

Speelmans G, Poolman B, Konings W . Amino acid transport in the thermophilic anaerobe Clostridium fervidus is driven by an electrochemical sodium gradient. J Bacteriol. 1993; 175(7):2060-6. PMC: 204302. DOI: 10.1128/jb.175.7.2060-2066.1993. View

11.

Skulachev V . Bacterial Na+ energetics. FEBS Lett. 1989; 250(1):106-14. DOI: 10.1016/0014-5793(89)80693-3. View

12.

Heyne R, de Vrij W, Crielaard W, Konings W . Sodium ion-dependent amino acid transport in membrane vesicles of Bacillus stearothermophilus. J Bacteriol. 1991; 173(2):791-800. PMC: 207073. DOI: 10.1128/jb.173.2.791-800.1991. View

13.

Wilson T, Lin E . Evolution of membrane bioenergetics. J Supramol Struct. 1980; 13(4):421-46. DOI: 10.1002/jss.400130403. View

14.

Speelmans G, Poolman B, Abee T, Konings W . Energy transduction in the thermophilic anaerobic bacterium Clostridium fervidus is exclusively coupled to sodium ions. Proc Natl Acad Sci U S A. 1993; 90(17):7975-9. PMC: 47270. DOI: 10.1073/pnas.90.17.7975. View

15.

de Rosa M, Gambacorta A, Gliozzi A . Structure, biosynthesis, and physicochemical properties of archaebacterial lipids. Microbiol Rev. 1986; 50(1):70-80. PMC: 373054. DOI: 10.1128/mr.50.1.70-80.1986. View

16.

Elferink M, de Wit J, Driessen A, Konings W . Stability and proton-permeability of liposomes composed of archaeal tetraether lipids. Biochim Biophys Acta. 1994; 1193(2):247-54. DOI: 10.1016/0005-2736(94)90160-0. View

17.

Lolkema J, Speelmans G, Konings W . Na(+)-coupled versus H(+)-coupled energy transduction in bacteria. Biochim Biophys Acta. 1994; 1187(2):211-5. DOI: 10.1016/0005-2728(94)90113-9. View

18.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

19.

Romano A, Brino G, Peterkofsky A, Reizer J . Regulation of beta-galactoside transport and accumulation in heterofermentative lactic acid bacteria. J Bacteriol. 1987; 169(12):5589-96. PMC: 213992. DOI: 10.1128/jb.169.12.5589-5596.1987. View