Inducible Phosphoenolpyruvate-dependent Hexose Phosphotransferase Activities in Escherichia Coli

Overview

Journal Biochem J

Specialty Biochemistry

Date 1972 Aug 1

PMID 4345358

Citations 62

Authors

H L Kornberg

R E Reeves

Affiliations

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Abstract

1. A method is described for measuring the rate of phosphoenolpyruvate-dependent phosphotransferase activity for a variety of hexoses in toluene-treated suspensions of Escherichia coli. 2. The specific activities of the phosphotransferases that catalyse the phosphorylation of hexoses are greatly affected by the carbon source for growth. 3. In all strains of E. coli tested, fructose phosphotransferase activity is induced by growth on fructose. 4. Strains of E. coli differ greatly in the rate at which they phosphorylate glucose, but all strains possess at least a low glucose phosphotransferase activity under any tested condition of growth. Glucose phosphotransferase activity is further induced by growth on glucose; this does not occur in a mutant that lacks the ability to take up methyl alpha-d-[(14)C]glucopyranoside and hence grows poorly on glucose. 5. When growing on fructose, two strains of E. coli synthesize the inducible glucose phosphotransferase system gratuitously, and to specific activities higher than observed during growth on glucose. A phosphotransferase catalysing the phosphorylation of mannose is similarly induced.

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References

Lin E . The genetics of bacterial transport systems. Annu Rev Genet. 1970; 4:225-62. DOI: 10.1146/annurev.ge.04.120170.001301. View

Ferenci T, Kornberg H . Role of fructose-1,6-diphosphatase in fructose utilization by Escherichia coli. FEBS Lett. 1971; 14(5):360-363. DOI: 10.1016/0014-5793(71)80301-0. View

KUNDIG W, Roseman S . Sugar transport. I. Isolation of a phosphotransferase system from Escherichia coli. J Biol Chem. 1971; 246(5):1393-406. View

Gachelin G . A new assay of the phosphotransferase system in Escherichia coli. Biochem Biophys Res Commun. 1969; 34(4):382-7. DOI: 10.1016/0006-291x(69)90392-1. View

Ferenci T, Kornberg H, Smith J . Isolation and properties of a regulatory mutant in the hexose phosphate transport system of Escherichia coli. FEBS Lett. 1971; 13(3):133-136. DOI: 10.1016/0014-5793(71)80218-1. View

FRAENKEL D . The phosphoenolpyruvate-initiated pathway of fructose metabolism in Escherichia coli. J Biol Chem. 1968; 243(24):6458-63. View

ASHWORTH J, Kornberg H . The anaplerotic fixation of carbon dioxide by Escherichia coli. Proc R Soc Lond B Biol Sci. 1966; 165(999):179-88. DOI: 10.1098/rspb.1966.0063. View

Kornberg H, Smith J . Genetic control of glucose uptake by Escherichia coli. FEBS Lett. 1972; 20(3):270-272. DOI: 10.1016/0014-5793(72)80084-x. View

Kornberg H, Reeves R . Correlation between hexose transport and phosphotransferase activity in Escherichia coli. Biochem J. 1972; 126(5):1241-3. PMC: 1178548. DOI: 10.1042/bj1261241. View

10.

KUNDIG W, Ghosh S, Roseman S . PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM. Proc Natl Acad Sci U S A. 1964; 52:1067-74. PMC: 300396. DOI: 10.1073/pnas.52.4.1067. View

11.

Brice C, Kornberg H . Location of a gene specifying phosphopyruvate synthase activity on the genome of Escherichia coli, K12. Proc R Soc Lond B Biol Sci. 1967; 168(1012):281-92. DOI: 10.1098/rspb.1967.0066. View

12.

Ferenci T, Kornberg H . Pathway of fructose utilization by Escherichia coli. FEBS Lett. 1971; 13(2):127-130. DOI: 10.1016/0014-5793(71)80216-8. View