Alpha 2.0
Login Register
» Articles » PMID: 9284180

A New Restriction Fragment Length Polymorphism from Cryptosporidium Parvum Identifies Genetically Heterogeneous Parasite Populations and Genotypic Changes Following Transmission from Bovine to Human Hosts

Overview
Journal Infect Immun
Publisher American Society for Microbiology
Specialty Allergy & Immunology
Date 1997 Sep 1
PMID 9284180
Citations 37
Authors
M Carraway
S Tzipori
G Widmer
Affiliations
Soon will be listed here.
Abstract

Length and restriction site polymorphism within a 2.8-kb threonine-rich open reading frame from Cryptosporidium parvum was identified and used to determine the genotypes of isolates from calves and humans. In agreement with observations of other genetic loci, all calf isolates were identical at this locus. In contrast, human isolates showed two profiles, one found exclusively in humans and one a superposition of both profiles, which were indicative of heterogeneous parasite populations. PCR fingerprints were consistent with a change in the genetic profile of C. parvum isolates following transmission from bovine to human hosts.

Citing Articles

Susceptibility of five strains of oocysts to UV light.

Clancy J, Marshall M, Hargy T, Korich D J Am Water Works Assoc. 2020; 96(3):84-93.

PMID: 32313290 PMC: 7159772. DOI: 10.1002/j.1551-8833.2004.tb10576.x.

Comparison of single- and multilocus genetic diversity in the protozoan parasites Cryptosporidium parvum and C. hominis.

Widmer G, Lee Y Appl Environ Microbiol. 2010; 76(19):6639-44.

PMID: 20709840 PMC: 2950454. DOI: 10.1128/AEM.01268-10.

Multi-locus analysis of human infective Cryptosporidium species and subtypes using ten novel genetic loci.

Bouzid M, Tyler K, Christen R, Chalmers R, Elwin K, Hunter P BMC Microbiol. 2010; 10:213.

PMID: 20696051 PMC: 2928199. DOI: 10.1186/1471-2180-10-213.

Silent information regulator 2 proteins encoded by Cryptosporidium parasites.

Yasukawa H, Yagita K Parasitol Res. 2010; 107(3):707-12.

PMID: 20563732 DOI: 10.1007/s00436-010-1925-8.

Detection of Cryptosporidium spp. from human faeces by PCR-RFLP, cloning and sequencing.

Leone A, Ripabelli G, Sammarco M, Grasso G Parasitol Res. 2008; 104(3):583-7.

PMID: 18979120 DOI: 10.1007/s00436-008-1233-8.

References
1.
Petersen C . Cryptosporidiosis in patients infected with the human immunodeficiency virus. Clin Infect Dis. 1992; 15(6):903-9. DOI: 10.1093/clind/15.6.903. View
2.
Tzipori S, Rand W, Griffiths J, Widmer G, Crabb J . Evaluation of an animal model system for cryptosporidiosis: therapeutic efficacy of paromomycin and hyperimmune bovine colostrum-immunoglobulin. Clin Diagn Lab Immunol. 1994; 1(4):450-63. PMC: 368287. DOI: 10.1128/cdli.1.4.450-463.1994. View
3.
Su X, Wellems T . Toward a high-resolution Plasmodium falciparum linkage map: polymorphic markers from hundreds of simple sequence repeats. Genomics. 1996; 33(3):430-44. DOI: 10.1006/geno.1996.0218. View
4.
Bonnin A, Fourmaux M, Dubremetz J, Nelson R, Gobet P, Harly G . Genotyping human and bovine isolates of Cryptosporidium parvum by polymerase chain reaction-restriction fragment length polymorphism analysis of a repetitive DNA sequence. FEMS Microbiol Lett. 1996; 137(2-3):207-11. DOI: 10.1111/j.1574-6968.1996.tb08107.x. View
5.
Pedersen C, Danner S, Lazzarin A, Glauser M, Weber R, Katlama C . Epidemiology of cryptosporidiosis among European AIDS patients. Genitourin Med. 1996; 72(2):128-31. PMC: 1195623. DOI: 10.1136/sti.72.2.128. View