Cryptosporidium Parvum Mixed Genotypes Detected by PCR-restriction Fragment Length Polymorphism Analysis

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2002 Jan 5

PMID 11772657

Citations 16

Authors

Carrie Reed

Gregory D Sturbaum

Paul J Hoover

Charles R Sterling

Affiliations

Soon will be listed here.

Abstract

Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.

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