Effect of Conjugated Linoleic Acid on Body Composition in Mice

Overview

Journal Lipids

Specialty Biochemistry

Date 1997 Aug 1

PMID 9270977

Citations 170

Authors

Y Park

K J Albright

W Liu

J M Storkson

M E Cook

M W Pariza

Affiliations

Soon will be listed here.

Abstract

The effects of conjugated linoleic acid (CLA) on body composition were investigated. ICR mice were fed a control diet containing 5.5% corn oil or a CLA-supplemented diet (5.0% corn oil plus 0.5% CLA). Mice fed CLA-supplemented diet exhibited 57% and 60% lower body fat and 5% and 14% increased lean body mass relative to controls (P < 0.05). Total carnitine palmitoyltransferase activity was increased by dietary CLA supplementation in both fat pad and skeletal muscle; the differences were significant for fat pad of fed mice and skeletal muscle of fasted mice. In cultured 3T3-L1 adipocytes CLA treatment (1 x 10(-4)M) significantly reduced heparin-releasable lipoprotein lipase activity (-66%) and the intracellular concentrations of triacylglyceride (-8%) and glycerol (-15%), but significantly increased free glycerol in the culture medium (+22%) compared to control (P < 0.05). The effects of CLA on body composition appear to be due in part to reduced fat deposition and increased lipolysis in adipocytes, possibly coupled with enhanced fatty acid oxidation in both muscle cells and adipocytes.

Citing Articles

The effect of conjugated linoleic acid supplementation in comparison with omega-6 and omega-9 on lipid profile: a graded, dose-response systematic review and meta-analysis of randomized controlled trials.

Akhgarjand C, Tavakoli A, Samavat S, Bagheri A, Anoushirvani A, Mirzababaei A Front Nutr. 2024; 11:1336889.

PMID: 38567248 PMC: 10985181. DOI: 10.3389/fnut.2024.1336889.

The effect of conjugated linoleic acids on inflammation, oxidative stress, body composition and physical performance: a comprehensive review of putative molecular mechanisms.

Putera H, Doewes R, Shalaby M, Ramirez-Coronel A, Clayton Z, Abdelbasset W Nutr Metab (Lond). 2023; 20(1):35.

PMID: 37644566 PMC: 10466845. DOI: 10.1186/s12986-023-00758-9.

Nutritional Considerations for the Vegan Athlete.

West S, Monteyne A, van der Heijden I, Stephens F, Wall B Adv Nutr. 2023; 14(4):774-795.

PMID: 37127187 PMC: 10334161. DOI: 10.1016/j.advnut.2023.04.012.

An investigation on conjugated linoleic acid content, fatty acid composition, and physicochemical characteristics of Iranian Kurdish butter oil.

Faraji Sarabmirza R, Joolaei Ahranjani P, Rashidi L, Mousavi M, Khodaiyan F, Nodeh H Food Sci Nutr. 2023; 11(2):1051-1058.

PMID: 36789035 PMC: 9922134. DOI: 10.1002/fsn3.3142.

Improving obesity and lipid metabolism using conjugated linoleic acid.

Sun Y, Hou X, Li L, Tang Y, Zheng M, Zeng W Vet Med Sci. 2022; 8(6):2538-2544.

PMID: 36104831 PMC: 9677407. DOI: 10.1002/vms3.921.

References

Beck S, Smith K, Tisdale M . Anticachectic and antitumor effect of eicosapentaenoic acid and its effect on protein turnover. Cancer Res. 1991; 51(22):6089-93. View

Hotamisligil G, Shargill N, Spiegelman B . Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science. 1993; 259(5091):87-91. DOI: 10.1126/science.7678183. View

Lokesh B, Hsieh H, Kinsella J . Peritoneal macrophages from mice fed dietary (n-3) polyunsaturated fatty acids secrete low levels of prostaglandins. J Nutr. 1986; 116(12):2547-52. DOI: 10.1093/jn/116.12.2547. View

Chin S, Storkson J, Albright K, Cook M, Pariza M . Conjugated linoleic acid is a growth factor for rats as shown by enhanced weight gain and improved feed efficiency. J Nutr. 1994; 124(12):2344-9. DOI: 10.1093/jn/124.12.344. View

Leslie C, Gonnerman W, Ullman M, Hayes K, Franzblau C, Cathcart E . Dietary fish oil modulates macrophage fatty acids and decreases arthritis susceptibility in mice. J Exp Med. 1985; 162(4):1336-49. PMC: 2187871. DOI: 10.1084/jem.162.4.1336. View

Parrish C, Pathy D, Angel A . Dietary fish oils limit adipose tissue hypertrophy in rats. Metabolism. 1990; 39(3):217-9. DOI: 10.1016/0026-0495(90)90038-e. View

Martyn P, HANSEN I . Initiation of lipogenic enzyme activities in rat mammary glands. Biochem J. 1981; 198(1):187-92. PMC: 1163225. DOI: 10.1042/bj1980187. View

Takada R, Saitoh M, Mori T . Dietary gamma-linolenic acid-enriched oil reduces body fat content and induces liver enzyme activities relating to fatty acid beta-oxidation in rats. J Nutr. 1994; 124(4):469-74. DOI: 10.1093/jn/124.4.469. View

Ip C, Chin S, Scimeca J, Pariza M . Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Res. 1991; 51(22):6118-24. View

10.

Plumb J, Milroy R, Kaye S . Effects of the pH dependence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide-formazan absorption on chemosensitivity determined by a novel tetrazolium-based assay. Cancer Res. 1989; 49(16):4435-40. View

11.

COHEN L, Choi K, Wang C . Influence of dietary fat, caloric restriction, and voluntary exercise on N-nitrosomethylurea-induced mammary tumorigenesis in rats. Cancer Res. 1988; 48(15):4276-83. View

12.

Frost S, Lane M . Evidence for the involvement of vicinal sulfhydryl groups in insulin-activated hexose transport by 3T3-L1 adipocytes. J Biol Chem. 1985; 260(5):2646-52. View

13.

Lee K, Kritchevsky D, Pariza M . Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis. 1994; 108(1):19-25. DOI: 10.1016/0021-9150(94)90034-5. View

14.

Bieber L, Abraham T, Helmrath T . A rapid spectrophotometric assay for carnitine palmitoyltransferase. Anal Biochem. 1972; 50(2):509-18. DOI: 10.1016/0003-2697(72)90061-9. View

15.

Hotamisligil G, Peraldi P, Budavari A, Ellis R, White M, Spiegelman B . IRS-1-mediated inhibition of insulin receptor tyrosine kinase activity in TNF-alpha- and obesity-induced insulin resistance. Science. 1996; 271(5249):665-8. DOI: 10.1126/science.271.5249.665. View

16.

Awad A, Bernardis L, Fink C . Failure to demonstrate an effect of dietary fatty acid composition on body weight, body composition and parameters of lipid metabolism in mature rats. J Nutr. 1990; 120(11):1277-82. DOI: 10.1093/jn/120.11.1277. View

17.

Nilsson-Ehle P, Schotz M . A stable, radioactive substrate emulsion for assay of lipoprotein lipase. J Lipid Res. 1976; 17(5):536-41. View

18.

Ha Y, Grimm N, Pariza M . Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis. 1987; 8(12):1881-7. DOI: 10.1093/carcin/8.12.1881. View

19.

Endres S, Ghorbani R, Kelley V, Georgilis K, Lonnemann G, van der Meer J . The effect of dietary supplementation with n-3 polyunsaturated fatty acids on the synthesis of interleukin-1 and tumor necrosis factor by mononuclear cells. N Engl J Med. 1989; 320(5):265-71. DOI: 10.1056/NEJM198902023200501. View

20.

Calder P, Bond J, Harvey D, Gordon S, Newsholme E . Uptake and incorporation of saturated and unsaturated fatty acids into macrophage lipids and their effect upon macrophage adhesion and phagocytosis. Biochem J. 1990; 269(3):807-14. PMC: 1131659. DOI: 10.1042/bj2690807. View