Dipeptidyl-peptidase IV Secreted by Aspergillus Fumigatus, a Fungus Pathogenic to Humans

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1997 Aug 1

PMID 9234752

Citations 29

Authors

A Beauvais

M Monod

J Wyniger

J P Debeaupuis

E Grouzmann

N Brakch

J Svab

A G Hovanessian

J P Latge

Affiliations

Soon will be listed here.

Abstract

A dipeptidyl-peptidase IV was purified from the culture medium of the human-pathogenic fungus Aspergillus fumigatus. The enzyme has an apparent molecular mass of 95 kDa and contained approximately 10 kDa of N-linked carbohydrate. This glycoprotein is antigenic and has all characteristics of the class IV dipeptidyl-peptidases: removal of Xaa-Pro and to a lesser extent Xaa-Ala dipeptides from the N termini of peptides, including bioactive peptides such as neuropeptide Y, [des-Arg1] bradykinin, and glucagon-like peptide 1, activity at neutral pH, and presence in the amino acid sequence of the Gly-X-Ser-X-Gly consensus motif of the serine-hydrolases and the putative catalytic triad (Ser613, Asp690, His725) of the dipeptidyl-peptidases. Moreover, the last 200 amino acids displayed 60 to 65% similarity with the other dipeptidyl-peptidases IV from rat, mouse, human, and yeast. However, unlike the other dipeptidyl-peptidases, the dipeptidyl-peptidase IV of A. fumigatus is a secreted enzyme with a cleavable signal peptide. Expression of a recombinant dipeptidyl-peptidase IV of A. fumigatus has been attained in the yeast Pichia pastoris.

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References

Cenci E, Perito S, Enssle K, Mosci P, Latge J, Romani L . Th1 and Th2 cytokines in mice with invasive aspergillosis. Infect Immun. 1997; 65(2):564-70. PMC: 176097. DOI: 10.1128/iai.65.2.564-570.1997. View

Pauly R, Rosche F, Wermann M, McIntosh C, PEDERSON R, Demuth H . Investigation of glucose-dependent insulinotropic polypeptide-(1-42) and glucagon-like peptide-1-(7-36) degradation in vitro by dipeptidyl peptidase IV using matrix-assisted laser desorption/ionization-time of flight mass spectrometry. A novel.... J Biol Chem. 1996; 271(38):23222-9. DOI: 10.1074/jbc.271.38.23222. View

Gorrell M, Wickson J, McCaughan G . Expression of the rat CD26 antigen (dipeptidyl peptidase IV) on subpopulations of rat lymphocytes. Cell Immunol. 1991; 134(1):205-15. DOI: 10.1016/0008-8749(91)90343-a. View

Kawasaki H, Emori Y, Suzuki K . Production and separation of peptides from proteins stained with Coomassie brilliant blue R-250 after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem. 1990; 191(2):332-6. DOI: 10.1016/0003-2697(90)90227-z. View

Calderone R, Braun P . Adherence and receptor relationships of Candida albicans. Microbiol Rev. 1991; 55(1):1-20. PMC: 372798. DOI: 10.1128/mr.55.1.1-20.1991. View

Latge J, Moutaouakil M, Debeaupuis J, Bouchara J, Haynes K, Prevost M . The 18-kilodalton antigen secreted by Aspergillus fumigatus. Infect Immun. 1991; 59(8):2586-94. PMC: 258060. DOI: 10.1128/iai.59.8.2586-2594.1991. View

Schon E, Born I, Demuth H, Faust J, Neubert K, Steinmetzer T . Dipeptidyl peptidase IV in the immune system. Effects of specific enzyme inhibitors on activity of dipeptidyl peptidase IV and proliferation of human lymphocytes. Biol Chem Hoppe Seyler. 1991; 372(5):305-11. DOI: 10.1515/bchm3.1991.372.1.305. View

Lamy B, Moutaouakil M, Latge J, Davies J . Secretion of a potential virulence factor, a fungal ribonucleotoxin, during human aspergillosis infections. Mol Microbiol. 1991; 5(7):1811-5. DOI: 10.1111/j.1365-2958.1991.tb01930.x. View

Marguet D, Bernard A, Vivier I, Darmoul D, Naquet P, Pierres M . cDNA cloning for mouse thymocyte-activating molecule. A multifunctional ecto-dipeptidyl peptidase IV (CD26) included in a subgroup of serine proteases. J Biol Chem. 1992; 267(4):2200-8. View

10.

Bauvois B, Sanceau J, Wietzerbin J . Human U937 cell surface peptidase activities: characterization and degradative effect on tumor necrosis factor-alpha. Eur J Immunol. 1992; 22(4):923-30. DOI: 10.1002/eji.1830220407. View

11.

Jaton-Ogay K, Suter M, Crameri R, Falchetto R, Fatih A, Monod M . Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease of Aspergillus fumigatus. FEMS Microbiol Lett. 1992; 71(2):163-8. DOI: 10.1016/0378-1097(92)90506-j. View

12.

Vanhoof G, De Meester I, Hendriks D, Goossens F, VAN SANDE M, Scharpe S . Proline-specific aminopeptidases: potential role in bradykinin degradation. Agents Actions Suppl. 1992; 38 ( Pt 2):120-7. View

13.

Kobayashi H, Debeaupuis J, Bouchara J, Latge J . An 88-kilodalton antigen secreted by Aspergillus fumigatus. Infect Immun. 1993; 61(11):4767-71. PMC: 281232. DOI: 10.1128/iai.61.11.4767-4771.1993. View

14.

Mentlein R, DAHMS P, Grandt D, Kruger R . Proteolytic processing of neuropeptide Y and peptide YY by dipeptidyl peptidase IV. Regul Pept. 1993; 49(2):133-44. DOI: 10.1016/0167-0115(93)90435-b. View

15.

Jaton-Ogay K, Paris S, Huerre M, Quadroni M, Falchetto R, Togni G . Cloning and disruption of the gene encoding an extracellular metalloprotease of Aspergillus fumigatus. Mol Microbiol. 1994; 14(5):917-28. DOI: 10.1111/j.1365-2958.1994.tb01327.x. View

16.

Chambers A, PACKHAM E, Graham I . Control of glycolytic gene expression in the budding yeast (Saccharomyces cerevisiae). Curr Genet. 1995; 29(1):1-9. DOI: 10.1007/BF00313187. View

17.

Jacotot E, Callebaut C, Blanco J, Krust B, Neubert K, Barth A . Dipeptidyl-peptidase IV-beta, a novel form of cell-surface-expressed protein with dipeptidyl-peptidase IV activity. Eur J Biochem. 1996; 239(2):248-58. DOI: 10.1111/j.1432-1033.1996.0248u.x. View

18.

Devereux J, Haeberli P, SMITHIES O . A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984; 12(1 Pt 1):387-95. PMC: 321012. DOI: 10.1093/nar/12.1part1.387. View

19.

Fox D, Hussey R, Fitzgerald K, Acuto O, Poole C, Palley L . Ta1, a novel 105 KD human T cell activation antigen defined by a monoclonal antibody. J Immunol. 1984; 133(3):1250-6. View

20.

Hanski C, Huhle T, Gossrau R, REUTTER W . Direct evidence for the binding of rat liver DPP IV to collagen in vitro. Exp Cell Res. 1988; 178(1):64-72. DOI: 10.1016/0014-4827(88)90378-3. View