Article: Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants

1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.

Citing Articles

Distribution of 3 alpha-hydroxysteroid dehydrogenase (bile acid binder) in rat small intestine: comparison with glutathione S-transferase subunits.

Yamamuro W, Stolz A, Takikawa H, Sugimoto M, Kaplowitz N J Gastroenterol. 1994; 29(2):115-9.

PMID: 8012502 DOI: 10.1007/BF02358670.

A comparison of erythrocyte glutathione S-transferase activity from human foetuses and adults.

Strange R, Johnston J, Coghill D, HUME R Biochem J. 1980; 188(2):475-9.

PMID: 7396875 PMC: 1161891. DOI: 10.1042/bj1880475.

Cholic acid binding by glutathione S-transferases from rat liver cytosol.

Hayes J, Strange R, PERCY-ROBB I Biochem J. 1980; 185(1):83-7.

PMID: 7378056 PMC: 1161272. DOI: 10.1042/bj1850083.

A study of the structures of the YaYa and YaYc glutathione S-transferases from rat liver cytosol. Evidence that the Ya monomer is responsible for lithocholate-binding activity.

Hayes J, Strange R, PERCY-ROBB I Biochem J. 1981; 197(2):491-502.

PMID: 7034719 PMC: 1163150. DOI: 10.1042/bj1970491.

The purification of the hepatic glutathione S-transferases of rainbow trout by glutathione affinity chromatography alters their isoelectric behaviour.

Ramage P, Nimmo I Biochem J. 1983; 211(2):523-6.

PMID: 6870845 PMC: 1154390. DOI: 10.1042/bj2110523.

References

1.

Tipping E, Ketterer B, Christodoulides L, ENDERBY G . The non-convalent binding of small molecules by ligandin. Interactions with steroids and their conjugates, fatty acids, bromosulphophthalein carcinogens, glutathione and realted compounds. Eur J Biochem. 1976; 67(2):583-90. DOI: 10.1111/j.1432-1033.1976.tb10724.x. View

2.

Strange R, Nimmo I, PERCY-ROBB I . Binding of bile acids by 100 000g supernatants from rat liver. Biochem J. 1977; 162(3):659-64. PMC: 1164650. DOI: 10.1042/bj1620659. View

3.

OMaille E, RICHARDS T, Short A . Factors determining the maximal rate of organic anion secretion by the liver and further evidence on the hepatic site of action of the hormone secretin. J Physiol. 1966; 186(2):424-38. PMC: 1395860. DOI: 10.1113/jphysiol.1966.sp008044. View

4.

Habig W, Pabst M, Fleischner G, GATMAITAN Z, Arias I, Jakoby W . The identity of glutathione S-transferase B with ligandin, a major binding protein of liver. Proc Natl Acad Sci U S A. 1974; 71(10):3879-82. PMC: 434288. DOI: 10.1073/pnas.71.10.3879. View

5.

Schwarz L, Burr R, Schwenk M, Pfaff E, Greim H . Uptake of taurocholic acid into isolated rat-liver cells. Eur J Biochem. 1975; 55(3):617-23. DOI: 10.1111/j.1432-1033.1975.tb02199.x. View

6.

Habig W, Pabst M, Jakoby W . Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 249(22):7130-9. View

7.

Kaplowitz N, PERCY-ROBB I, JAVITT N . Role of hepatic anion-binding protein in bromsulphthalein conjugation. J Exp Med. 1973; 138(2):483-7. PMC: 2139406. DOI: 10.1084/jem.138.2.483. View

8.

Levi A, GATMAITAN Z, Arias I . Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions. J Clin Invest. 1969; 48(11):2156-67. PMC: 297469. DOI: 10.1172/JCI106182. View

9.

Litwack G, Ketterer B, Arias I . Ligandin: a hepatic protein which binds steroids, bilirubin, carcinogens and a number of exogenous organic anions. Nature. 1971; 234(5330):466-7. DOI: 10.1038/234466a0. View

10.

Strange R, Nimmo I, PERCY-ROBB I . Equilibrium-dialysis studies of the interaction between cholic acid and 100000g-supernatant preparations from the rat liver. Biochem J. 1976; 156(2):427-33. PMC: 1163764. DOI: 10.1042/bj1560427. View

11.

Erlinger S . Hepatocellular uptake of taurocholate in the dog. J Clin Invest. 1975; 55(2):419-26. PMC: 301761. DOI: 10.1172/JCI107946. View

12.

Okishio T, Nair P . Studies on bile acids. Some observations on the intracellular localization of major bile acids in rat liver. Biochemistry. 1966; 5(11):3662-8. DOI: 10.1021/bi00875a040. View

Partial Purification of Two Lithocholic Acid-binding Proteins from Rat Liver 100 000g Supernatants