Binding of Bile Acids by 100 000g Supernatants from Rat Liver

Overview

Journal Biochem J

Specialty Biochemistry

Date 1977 Mar 15

PMID 869910

Citations 9

Authors

R C Strange

I A Nimmo

I W PERCY-ROBB

Affiliations

Soon will be listed here.

Abstract

1. The binding of glycocholic acid, chenodeoxycholic acid and lithocholic acid to rat liver 1000 000g supernatants was studied by equilibrium dialysis. 2. The binding characteristics of the bile acids suggest that the binding components are involved in bile acid transport. 3. When mixtures of [14C]lithocholic acid and liver supernatants were eluted from columns of Sephadex G-75, a prominent peak of [14C]lithocholic acid appeared with proteins of mol.wt. approx. 40000. A second, smaller, peak of [14C]lithocholic acid was eluted with proteins of mol.wt. approx. 100000. 4. The inclusion of cholic acid, glycocholic acid or chenodeoxycholic acid in the eluting buffer decreased the amount of [14C]lithocholic acid that was eluted with the higher-molecular-weight component.

Citing Articles

A Cytokinin-binding Protein from Wheat Germ: Isolation by Affinity Chromatography and Properties.

MOORE F Plant Physiol. 1979; 64(4):594-9.

PMID: 16661016 PMC: 543144. DOI: 10.1104/pp.64.4.594.

Cholic acid binding by glutathione S-transferases from rat liver cytosol.

Hayes J, Strange R, PERCY-ROBB I Biochem J. 1980; 185(1):83-7.

PMID: 7378056 PMC: 1161272. DOI: 10.1042/bj1850083.

Is ligandin relevant for the uptake and storage of phallotoxins in liver cells?.

Ziegler K, Grundmann E, Veil L, Frimmer M Naunyn Schmiedebergs Arch Pharmacol. 1981; 317(4):364-7.

PMID: 7322218 DOI: 10.1007/BF00501320.

[Pathogenic significance of bile acids (author's transl)].

Gerok W, Matern S Klin Wochenschr. 1981; 59(12):575-89.

PMID: 7253534 DOI: 10.1007/BF02593847.

Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants.

Strange R, Cramb R, Hayes J, PERCY-ROBB I Biochem J. 1977; 165(3):425-9.

PMID: 921757 PMC: 1164923. DOI: 10.1042/bj1650425.

References

Hofmann A . THE FUNCTION OF BILE SALTS IN FAT ABSORPTION. THE SOLVENT PROPERTIES OF DILUTE MICELLAR SOLUTIONS OF CONJUGATED BILE SALTS. Biochem J. 1963; 89:57-68. PMC: 1202272. DOI: 10.1042/bj0890057. View

Eriksson S . Biliary excretion of bile acids and cholesterol in bile fistula rats; bile acids and steroids. Proc Soc Exp Biol Med. 1957; 94(3):578-82. DOI: 10.3181/00379727-94-23018. View

Rousseau G, Baxter J, Tomkins G . Glucocorticoid receptors: relations between steroid binding and biological effects. J Mol Biol. 1972; 67(1):99-115. DOI: 10.1016/0022-2836(72)90389-0. View

BOYD G, Grimwade A, Lawson M . Studies on rat-liver microsomal cholesterol 7alpha-hydroxylase. Eur J Biochem. 1973; 37(2):334-40. DOI: 10.1111/j.1432-1033.1973.tb02992.x. View

JENSEN E, Desombre E . Mechanism of action of the female sex hormones. Annu Rev Biochem. 1972; 41:203-30. DOI: 10.1146/annurev.bi.41.070172.001223. View