Genetic Analysis of Interactions Between Gag Proteins of Rous Sarcoma Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1997 Jul 1

PMID 9188638

Citations 8

Authors

X Li

B Yuan

S P Goff

Affiliations

Soon will be listed here.

Abstract

The yeast two-hybrid system was used to characterize homomeric interactions between the Gag proteins of Rous sarcoma virus (RSV). The RSV Gag precursor was found to interact strongly with itself and not with various control proteins. The RSV Gag did not interact significantly with Gag proteins of a variety of other retroviruses, including murine leukemia viruses and primate lentiviruses. Deletion analysis suggested that two nonoverlapping regions are independently sufficient to mediate RSV Gag-Gag dimerization. One such region lies near the N terminus and contains p2, p10, and a large N-terminal part of the capsid (CA) domain; the other is localized in the C terminus and includes a small C-terminal portion of CA and the nucleocapsid protein. These interaction domains may play roles in viral assembly.

Citing Articles

Intermolecular interactions between retroviral Gag proteins in the nucleus.

Kenney S, Lochmann T, Schmid C, Parent L J Virol. 2007; 82(2):683-91.

PMID: 17977961 PMC: 2224582. DOI: 10.1128/JVI.02049-07.

The C-terminal portion of the Hrs protein interacts with Tsg101 and interferes with human immunodeficiency virus type 1 Gag particle production.

Bouamr F, Houck-Loomis B, De Los Santos M, Casaday R, Johnson M, Goff S J Virol. 2006; 81(6):2909-22.

PMID: 17182674 PMC: 1865988. DOI: 10.1128/JVI.01413-06.

Basic residues of the retroviral nucleocapsid play different roles in gag-gag and Gag-Psi RNA interactions.

Lee E, Linial M J Virol. 2004; 78(16):8486-95.

PMID: 15280457 PMC: 479049. DOI: 10.1128/JVI.78.16.8486-8495.2004.

Endophilins interact with Moloney murine leukemia virus Gag and modulate virion production.

Wang M, Kim W, Gao G, Torrey T, Morse 3rd H, De Camilli P J Biol. 2003; 3(1):4.

PMID: 14659004 PMC: 442166. DOI: 10.1186/1475-4924-3-4.

The carboxy-terminal fragment of nucleolin interacts with the nucleocapsid domain of retroviral gag proteins and inhibits virion assembly.

Bacharach E, Gonsky J, ALIN K, Orlova M, Goff S J Virol. 2000; 74(23):11027-39.

PMID: 11069998 PMC: 113183. DOI: 10.1128/jvi.74.23.11027-11039.2000.

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