Specificity and Sequence Requirements for Interactions Between Various Retroviral Gag Proteins

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1994 Aug 1

PMID 8035530

Citations 57

Authors

E K FRANKE

H E Yuan

K L Bossolt

S P Goff

J LUBAN

Affiliations

Soon will be listed here.

Abstract

We previously established a genetic assay for retroviral Gag polyprotein multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S. P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to demonstrate homomeric interactions between Gag polyproteins encoded by six different retroviruses. Of the Gag polyproteins tested, only those encoded by closely related retroviruses formed heteromultimers. To determine the primary sequence requirements for human immunodeficiency virus type 1 Gag polyprotein multimerization, we studied the effects on multimerization of deletion and linker insertion mutations. Sequences necessary for this process were located between the C-terminal one-third of the capsid domain and the C terminus of the nucleocapsid domain.

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