Characterization and Crystallization of the Helicase Domain of Bacteriophage T7 Gene 4 Protein

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1997 Jul 1

PMID 9185573

Citations 25

Authors

L E BIRD

K Hakansson

H Pan

D B Wigley

Affiliations

Soon will be listed here.

Abstract

Limited proteolysis of bacteriophage T7 primase/helicase with endoproteinase Glu-C produces several proteolytic fragments. One of these fragments, which is derived from the C-terminal region of the protein, was prepared and shown to retain helicase activity. This result supports a model in which the gene 4 proteins consist of functionally separable domains. Crystals of this C-terminal fragment of the protein have been obtained that are suitable for X-ray diffraction studies.

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