Transient Aggregates in Protein Folding Are Easily Mistaken for Folding Intermediates

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1997 Jun 10

PMID 9177173

Citations 53

Authors

M Silow

M Oliveberg

Affiliations

Soon will be listed here.

Abstract

It has been questioned recently whether populated intermediates are important for the protein folding process or are artefacts trapped in nonproductive pathways. We report here that the rapidly formed intermediate of the spliceosomal protein U1A is an off-pathway artefact caused by transient aggregation of denatured protein under native conditions. Transient aggregates are easily mistaken for structured monomers and could be a general problem in time-resolved folding studies.

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