Antineutrophil Cytoplasm Autoantibodies Against Bactericidal/permeability-increasing Protein in Inflammatory Bowel Disease

Overview

Journal Gut

Specialty Gastroenterology

Date 1997 Jan 1

PMID 9155585

Citations 23

Authors

R S Walmsley

M H Zhao

M I Hamilton

A Brownlee

P Chapman

R E Pounder

A J Wakefield

C M Lockwood

Affiliations

Soon will be listed here.

Abstract

Background: Bactericidal/permeability-increasing protein (BPI), a constituent of primary neutrophil granules, is a potent natural antibiotic and an antineutrophil cytoplasm antibody (ANCA) antigen in cases of vasculitis in which the target antigen is neither myeloperoxidase (MPO) nor proteinase-3 (PR3).

Aim: To investigate BPI as a possible target antigen for ANCAs in inflammatory bowel disease.

Methods: ANCAs were detected by routine immunofluorescence (IIF) and solid phase enzyme linked immunosorbent assay (ELISA) performed for antibodies to the purified neutrophil granule proteins; MPO, PR3, cathepsin-G, lactoferrin, and BPI in serum samples from 88 patients with inflammatory bowel disease (36 with Crohn's disease, 52 with ulcerative colitis). Thirty patients with bacterial enteritis acted as controls.

Results: Significantly more patients with ulcerative colitis were ANCA positive by IIF (60%) than patients with Crohn's disease (28%) or infectious enteritis (23%) (p < 0.001). IgG anti-BPI antibodies were present in 29% of patients with ulcerative colitis, 14% of patients with Crohn's disease, and 23% of patients with infectious enteritis, occurring in 44% of those patients with inflammatory bowel disease who were ANCA positive by IIF. Antibodies to other ANCA antigens were rare. The presence of ANCAs was not related to either disease activity or extent; presence of anti-BPI antibodies was significantly related to both a lower serum albumin concentration (p = 0.001) and a higher erythrocyte sedimentation rate (p = 0.02) in patients with ulcerative colitis, and to colonic involvement in patients with Crohn's disease (p = 0.01).

Conclusion: BPI is a significant minority target antigen for ANCAs in inflammatory bowel disease that seems related to colonic Crohn's disease and disease activity in ulcerative colitis. Anti-BPI antibodies occur in infectious enteritis.

Citing Articles

Plasma levels of bactericidal/permeability-increasing protein correlate with systemic inflammation in acute coronary syndrome.

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Differential Enhancement of Neutrophil Phagocytosis by Anti-Bactericidal/Permeability-Increasing Protein Antibodies.

Theprungsirikul J, Skopelja-Gardner S, Wierzbicki R, Sessions K, Rigby W J Immunol. 2021; 207(3):777-783.

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Killing three birds with one BPI: Bactericidal, opsonic, and anti-inflammatory functions.

Theprungsirikul J, Skopelja-Gardner S, Rigby W J Transl Autoimmun. 2021; 4:100105.

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Bactericidal Permeability Increasing Protein Deficiency Aggravates Acute Colitis in Mice by Increasing the Serum Levels of Lipopolysaccharide.

Kong Q, Lv Z, Kang Y, An Y, Liu Z, Zhang J Front Immunol. 2021; 11:614169.

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Low-Avidity Autoantibodies against Bactericidal/Permeability-Increasing Protein Occur in Gram-Negative and Gram-Positive Bacteremia.

Theprungsirikul J, Thaden J, Wierzbicki R, Burns A, Skopelja-Gardner S, Fowler Jr V Infect Immun. 2020; 88(10).

PMID: 32747603 PMC: 7504969. DOI: 10.1128/IAI.00444-20.

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