Bactericidal Permeability Increasing Protein Gene Polymorphism is Associated with Inflammatory Bowel Diseases in the Turkish Population

Overview

Journal Saudi J Gastroenterol

Specialty Gastroenterology

Date 2015 Aug 1

PMID 26228368

Citations 3

Authors

Guray Can

Hakan Akin

Filiz T Ozdemir

Hatice Can

Bulent Yilmaz

Fatih Eren

Ozlen Atug

Belkis Unsal

Hulya O Hamzaoglu

Affiliations

Soon will be listed here.

Abstract

Background/aims: Inflammatory bowel disease, a chronic inflammatory disease with unknown etiology, affects the small and large bowel at different levels. It is increasingly considered that innate immune system may have a central position in the pathogenesis of the disease. As a part of the innate immune system, bactericidal permeability increasing protein has an important role in the recognition and neutralization of gram-negative bacteria. The aim of our study was to investigate the involvement of bactericidal permeability increasing protein gene polymorphism (bactericidal permeability increasing protein Lys216Glu) in inflammatory bowel disease in a large group of Turkish patients.

Patients And Methods: The present study included 528 inflammatory bowel disease patients, 224 with Crohn's disease and 304 with ulcerative colitis, and 339 healthy controls.

Results: Bactericidal permeability increasing protein Lys216Glu polymorphism was found to be associated with both Crohn's disease and ulcerative colitis (P = 0.0001). The frequency of the Glu/Glu genotype was significantly lower in patients using steroids and in those with steroid dependence (P = 0.012, OR, 0.80; 95% confidence interval [CI]: 0.68-0.94; P = 0.0286, OR, 0.75; 95% CI: 0.66-0.86, respectively). There was no other association between bactericidal permeability increasing protein gene polymorphism and phenotypes of inflammatory bowel disease.

Conclusions: Bactericidal permeability increasing protein Lys216Glu polymorphism is associated with both Crohn's disease and ulcerative colitis. This is the first study reporting the association of bactericidal permeability increasing protein gene polymorphism with steroid use and dependence in Crohn's disease.

Citing Articles

Killing three birds with one BPI: Bactericidal, opsonic, and anti-inflammatory functions.

Theprungsirikul J, Skopelja-Gardner S, Rigby W J Transl Autoimmun. 2021; 4:100105.

PMID: 34142075 PMC: 8187252. DOI: 10.1016/j.jtauto.2021.100105.

Bactericidal Permeability Increasing Protein Deficiency Aggravates Acute Colitis in Mice by Increasing the Serum Levels of Lipopolysaccharide.

Kong Q, Lv Z, Kang Y, An Y, Liu Z, Zhang J Front Immunol. 2021; 11:614169.

PMID: 33552078 PMC: 7858664. DOI: 10.3389/fimmu.2020.614169.

Bactericidal/permeability increasing protein gene polymorphism and inflammatory bowel diseases: meta-analysis of five case-control studies.

Fan L, Fu G, Ding Y, Lv P, Li H Int J Colorectal Dis. 2016; 32(3):433-435.

PMID: 28035462 DOI: 10.1007/s00384-016-2740-1.

References

van der Schaft D, Toebes E, Haseman J, Mayo K, Griffioen A . Bactericidal/permeability-increasing protein (BPI) inhibits angiogenesis via induction of apoptosis in vascular endothelial cells. Blood. 2000; 96(1):176-81. View

Arbour N, Lorenz E, Schutte B, Zabner J, Kline J, Jones M . TLR4 mutations are associated with endotoxin hyporesponsiveness in humans. Nat Genet. 2000; 25(2):187-91. DOI: 10.1038/76048. View

Ogura Y, Bonen D, Inohara N, Nicolae D, Chen F, Ramos R . A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease. Nature. 2001; 411(6837):603-6. DOI: 10.1038/35079114. View

Hampe J, Cuthbert A, Croucher P, Mirza M, Mascheretti S, Fisher S . Association between insertion mutation in NOD2 gene and Crohn's disease in German and British populations. Lancet. 2001; 357(9272):1925-8. DOI: 10.1016/S0140-6736(00)05063-7. View

Hendrickson B, Gokhale R, Cho J . Clinical aspects and pathophysiology of inflammatory bowel disease. Clin Microbiol Rev. 2002; 15(1):79-94. PMC: 118061. DOI: 10.1128/CMR.15.1.79-94.2002. View

Levy O, Canny G, Serhan C, Colgan S . Expression of BPI (bactericidal/permeability-increasing protein) in human mucosal epithelia. Biochem Soc Trans. 2003; 31(Pt 4):795-800. DOI: 10.1042/bst0310795. View

Torok H, Glas J, Tonenchi L, Mussack T, Folwaczny C . Polymorphisms of the lipopolysaccharide-signaling complex in inflammatory bowel disease: association of a mutation in the Toll-like receptor 4 gene with ulcerative colitis. Clin Immunol. 2004; 112(1):85-91. DOI: 10.1016/j.clim.2004.03.002. View

Ooi C, Weiss J, Elsbach P, Frangione B, Mannion B . A 25-kDa NH2-terminal fragment carries all the antibacterial activities of the human neutrophil 60-kDa bactericidal/permeability-increasing protein. J Biol Chem. 1987; 262(31):14891-4. View

John S, WEITZNER G, Rozen R, Scriver C . A rapid procedure for extracting genomic DNA from leukocytes. Nucleic Acids Res. 1991; 19(2):408. PMC: 333617. DOI: 10.1093/nar/19.2.408. View

10.

Ooi C, Weiss J, Doerfler M, Elsbach P . Endotoxin-neutralizing properties of the 25 kD N-terminal fragment and a newly isolated 30 kD C-terminal fragment of the 55-60 kD bactericidal/permeability-increasing protein of human neutrophils. J Exp Med. 1991; 174(3):649-55. PMC: 2118937. DOI: 10.1084/jem.174.3.649. View

11.

Monajemi H, Meenan J, Lamping R, Obradov D, Radema S, TROWN P . Inflammatory bowel disease is associated with increased mucosal levels of bactericidal/permeability-increasing protein. Gastroenterology. 1996; 110(3):733-9. DOI: 10.1053/gast.1996.v110.pm8608882. View

12.

Hilsden R, Meddings J, Sutherland L . Intestinal permeability changes in response to acetylsalicylic acid in relatives of patients with Crohn's disease. Gastroenterology. 1996; 110(5):1395-403. DOI: 10.1053/gast.1996.v110.pm8613043. View

13.

Macpherson A, Khoo U, Forgacs I, Philpott-Howard J, Bjarnason I . Mucosal antibodies in inflammatory bowel disease are directed against intestinal bacteria. Gut. 1996; 38(3):365-75. PMC: 1383064. DOI: 10.1136/gut.38.3.365. View

14.

Walmsley R, Zhao M, Hamilton M, Brownlee A, Chapman P, Pounder R . Antineutrophil cytoplasm autoantibodies against bactericidal/permeability-increasing protein in inflammatory bowel disease. Gut. 1997; 40(1):105-9. PMC: 1027017. DOI: 10.1136/gut.40.1.105. View

15.

Haapamaki M, Haggblom J, Gronroos J, Pekkala E, Alanen K, Nevalainen T . Bactericidal/permeability-increasing protein in colonic mucosa in ulcerative colitis. Hepatogastroenterology. 1999; 46(28):2273-7. View

16.

Klein W, Tromm A, Folwaczny C, Hagedorn M, Duerig N, Epplen J . A polymorphism of the bactericidal/permeability increasing protein (BPI) gene is associated with Crohn's disease. J Clin Gastroenterol. 2005; 39(4):282-3. DOI: 10.1097/01.mcg.0000155127.20290.07. View

17.

Lakatos P, Fischer S, Lakatos L, Gal I, Papp J . Current concept on the pathogenesis of inflammatory bowel disease-crosstalk between genetic and microbial factors: pathogenic bacteria and altered bacterial sensing or changes in mucosal integrity take "toll" ?. World J Gastroenterol. 2006; 12(12):1829-41. PMC: 4087507. DOI: 10.3748/wjg.v12.i12.1829. View

18.

Uyar F, Over-Hamzaoglu H, Ture F, Gul A, Tozun N, Saruhan-Direskeneli G . Distribution of common CARD15 variants in patients with sporadic Crohn's disease: cases from Turkey. Dig Dis Sci. 2006; 51(4):706-10. DOI: 10.1007/s10620-006-3195-y. View

19.

Petermann I, Huebner C, Browning B, Gearry R, Barclay M, Kennedy M . Interactions among genes influencing bacterial recognition increase IBD risk in a population-based New Zealand cohort. Hum Immunol. 2009; 70(6):440-6. DOI: 10.1016/j.humimm.2009.03.002. View

20.

Akin H, Tahan G, Ture F, Eren F, Atug O, Tahan V . Association between bactericidal/permeability increasing protein (BPI) gene polymorphism (Lys216Glu) and inflammatory bowel disease. J Crohns Colitis. 2011; 5(1):14-8. DOI: 10.1016/j.crohns.2010.08.008. View