Cloning and Sequence Analysis of a Class A Beta-lactamase from Mycobacterium Tuberculosis H37Ra

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1997 May 1

PMID 9145897

Citations 24

Authors

C J Hackbarth

I Unsal

H F Chambers

Affiliations

Soon will be listed here.

Abstract

A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype.

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