Characterization of Smooth Lipopolysaccharides and O Polysaccharides of Brucella Species by Competition Binding Assays with Monoclonal Antibodies

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1997 May 1

PMID 9125584

Citations 17

Authors

V Weynants

D Gilson

A Cloeckaert

A Tibor

P A Denoel

F Godfroid

J N Limet

J J Letesson

Affiliations

Soon will be listed here.

Abstract

Previously, four epitope specificities on the O chain of Brucella species were reported: M, A, C, and C/Y. In this work, according to monoclonal antibody binding to smooth lipopolysaccharides of Yersinia enterocolitica 0:9, Brucella abortus W99 (A-dominant strain), and B. melitensis Rev1 (M-dominant strain), seven O-chain epitope specificities were defined: M, A, C (M > A), C (M = A), C/Y (M > A), C/Y (M = A) and C/Y (A > M). Competitive binding assays between these monoclonal antibodies suggested that these different epitopes are probably overlapping structures.

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