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» Articles » PMID: 9115373

Precise Branch Point Mapping and Quantification of Splicing Intermediates

Overview
Journal Nucleic Acids Res
Publisher Oxford University Press
Specialty Biochemistry
Date 1997 May 15
PMID 9115373
Citations 62
Authors
J Vogel
W R Hess
T Borner
Affiliations
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Abstract

Lariat intermediates of a group II intron were investigated via RT-PCR. Several reverse transcriptases appeared capable of reading through a branched nucleotide. A new method has been established that yields precise information about the location of the branch point within an intron. As an extension of our approach, antisense transcripts of the previously cloned PCR products were successfully used in RNase Protection Assays, providing a tool for quantification of splicing intermediates. Application of the method presented to other self-splicing introns as well as introns in nuclear pre-mRNAs is envisaged.

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References
1.
Domdey H, Apostol B, Lin R, Newman A, BRODY E, Abelson J . Lariat structures are in vivo intermediates in yeast pre-mRNA splicing. Cell. 1984; 39(3 Pt 2):611-21. DOI: 10.1016/0092-8674(84)90468-9. View
2.
Myers R, Larin Z, Maniatis T . Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes. Science. 1985; 230(4731):1242-6. DOI: 10.1126/science.4071043. View
3.
Padgett R, Konarska M, Aebi M, HORNIG H, Weissmann C, Sharp P . Nonconsensus branch-site sequences in the in vitro splicing of transcripts of mutant rabbit beta-globin genes. Proc Natl Acad Sci U S A. 1985; 82(24):8349-53. PMC: 390913. DOI: 10.1073/pnas.82.24.8349. View
4.
Michel F, Umesono K, Ozeki H . Comparative and functional anatomy of group II catalytic introns--a review. Gene. 1989; 82(1):5-30. DOI: 10.1016/0378-1119(89)90026-7. View
5.
Hubschmann T, Hess W, Borner T . Impaired splicing of the rps12 transcript in ribosome-deficient plastids. Plant Mol Biol. 1996; 30(1):109-23. DOI: 10.1007/BF00017806. View