Epitope Mapping of a Monoclonal Antibody Specific to Feline Panleukopenia Virus and Mink Enteritis Virus

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 1997 Feb 1

PMID 9070987

Citations 6

Authors

M Horiuchi

M Mochizuki

N Ishiguro

H Nagasawa

M Shinagawa

Affiliations

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Abstract

To obtain monoclonal antibodies (MAbs) specific to feline panleukopenia virus (FPLV) and mink enteritis virus (MEV), 15 hybridomas secreting MAbs against MEV-Abashiri were established and the properties of the MAbs were analyzed. The cross-reactivity of MAbs revealed that one MAb, P2-215 was specific for FPLV and MEV, whereas the remaining fourteen MAbs reacted with canine parvovirus (CPV), FPLV, and MEV. Epitope analyses using various CPV/MEV chimeric viruses revealed that the MAb P2-215 recognized the epitope comprised of amino acid 93-Lys in VP2, which is known to be FPLV and MEV-specific.

Citing Articles

Enhancement of binding avidity by bivalent binding enables PrPSc-specific detection by anti-PrP monoclonal antibody 132.

Suzuki A, Yamasaki T, Hasebe R, Horiuchi M PLoS One. 2019; 14(6):e0217944.

PMID: 31170247 PMC: 6553756. DOI: 10.1371/journal.pone.0217944.

Flow Cytometric Detection of PrP in Neurons and Glial Cells from Prion-Infected Mouse Brains.

Yamasaki T, Suzuki A, Hasebe R, Horiuchi M J Virol. 2017; 92(1).

PMID: 29046463 PMC: 5730779. DOI: 10.1128/JVI.01457-17.

Establishment of a simple cell-based ELISA for the direct detection of abnormal isoform of prion protein from prion-infected cells without cell lysis and proteinase K treatment.

Shan Z, Yamasaki T, Suzuki A, Hasebe R, Horiuchi M Prion. 2016; 10(4):305-18.

PMID: 27565564 PMC: 5082968. DOI: 10.1080/19336896.2016.1189053.

Comparison of abnormal isoform of prion protein in prion-infected cell lines and primary-cultured neurons by PrPSc-specific immunostaining.

Tanaka M, Fujiwara A, Suzuki A, Yamasaki T, Hasebe R, Masujin K J Gen Virol. 2016; 97(8):2030-2042.

PMID: 27267758 PMC: 5770843. DOI: 10.1099/jgv.0.000514.

Comparison of biological and genomic characteristics between a newly isolated mink enteritis parvovirus MEV-LHV and an attenuated strain MEV-L.

Mao Y, Wang J, Hou Q, Xi J, Zhang X, Bian D Virus Genes. 2016; 52(3):388-96.

PMID: 26993137 DOI: 10.1007/s11262-016-1314-1.