Faster Synthesis and Slower Degradation of Liver Protein During Developmental Growth

Overview

Journal Biochem J

Specialty Biochemistry

Date 1977 Jul 15

PMID 901411

Citations 11

Authors

R D Conde

O A SCORNIK

Affiliations

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Abstract

A study is presented of the liver protein gain during the early stages of postnatal development. Fractional rates of protein synthesis and degradation were determined in vivo in livers of 4-day-old mice. At this age, liver protein accumulated at a rate of 18% per day. Synthesis was measured after the injection of massive amounts of radioactive leucine. Degradation was extimated as the balance between synthesis and accumulation of stable liver proteins, or from the disappearance of radioactivity from liver protein previously labelled by the administration of NaH14CO3. We found that the neonatal livers: (1) synthesize 139% as much protein per unit time and unit mass as adult tissue, which is accounted for by a higher ribosome concentration (synthesis per mg of RNA was the same); (2) retain 39% of the newly synthesized protein as stable liver components (compared with 48% in adult mice); (3) degrade protein at 56% of the rate in the adult liver. This lower rate of degradation is quantitatively the most significant difference between the growing and non-growing liver.

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References

Conde R, SCORNIK O . Role of protein degradation in the growth of livers after a nutritional shift. Biochem J. 1976; 158(2):385-90. PMC: 1163981. DOI: 10.1042/bj1580385. View

Miller S . Effect of starvation on protein synthesis in neonatal rat liver. J Nutr. 1975; 105(10):1326-33. DOI: 10.1093/jn/105.10.1326. View

SCORNIK O, Botbol V . Role of changes in protein degradation in the growth of regenerating livers. J Biol Chem. 1976; 251(10):2891-7. View

SCORNIK O . In vivo rates of deaggregation of polyribosomes in normal and regenerating liver after the injection of pactamycin. Biochim Biophys Acta. 1974; 374(1):76-81. DOI: 10.1016/0005-2787(74)90200-7. View

SCORNIK O . In vivo rate of translation by ribosomes of normal and regenerating liver. J Biol Chem. 1974; 249(12):3876-83. View

Drotman R, Campbell J . Protein arginine biosynthesis by mammalian liver tissue during postnatal development. Am J Physiol. 1972; 222(5):1204-12. DOI: 10.1152/ajplegacy.1972.222.5.1204. View

Otten J . Rat liver polyribosomes during the first days of life. Growth. 1968; 32(2):95-102. View

Richmond J, Shoemaker W, Elwyn D . Rates of biosynthesis of plasma and liver proteins. Am J Physiol. 1963; 205(5):848-56. DOI: 10.1152/ajplegacy.1963.205.5.848. View

Conconi F, Bank A, Marks P . Polyribosomes and control of protein synthesis: effects of sodium fluoride and temperature of reticulocytes. J Mol Biol. 1966; 19(2):525-40. DOI: 10.1016/s0022-2836(66)80020-7. View

10.

SCHREIER K, PORATH U, Schneider M, Weber M, Mehra K, PLUECKTHUN H . [Studies on the developmental physiology of protein metabolism in different organs of the rabbit]. Clin Chim Acta. 1961; 6:205-28. DOI: 10.1016/0009-8981(61)90086-9. View

11.

WIDDOWSON E, McCance R . Some effects of accelerating growth. I. General somatic development. Proc R Soc Lond B Biol Sci. 1960; 152:188-206. DOI: 10.1098/rspb.1960.0032. View

12.

Raiha N, Suihkonen J . Factors influencing the development of urea-synthesizing enzymes in rat liver. Biochem J. 1968; 107(6):793-7. PMC: 1198750. DOI: 10.1042/bj1070793. View

13.

Hod Y, Hershko A . Relationship of the pool of intracellular valine to protein synthesis and degradation in cultured cells. J Biol Chem. 1976; 251(14):4458-7. View

14.

Khairallah E, MORTIMORE G . Assessment of protein turnover in perfused rat liver. Evidence for amino acid compartmentation from differential labeling of free and tRNA-gound valine. J Biol Chem. 1976; 251(5):1375-84. View

15.

SWICK R, Ip M . Measurement of protein turnover in rat liver with (14C)carbonate. Protein turnover during liver regeneration. J Biol Chem. 1974; 249(21):6836-41. View

16.

Rosso P, Nelson M, Winick M . Changes in cellular RNA content and alkaline ribonuclease activity in rat liver during development. Growth. 1973; 37(2):143-51. View

17.

ROHR H, Wirz A, Henning L, Riede U, Bianchi L . Morphometric analysis of the rat liver cell in the perinatal period. Lab Invest. 1971; 24(2):128-39. View

18.

Illnerova H . Urea formation in rats during postnatal development. Biol Neonat. 1965; 9(1):197-202. DOI: 10.1159/000239990. View

19.

Winick M, Noble A . Quantitative changes in DNA, RNA, and protein during prenatal and postnatal growth in the rat. Dev Biol. 1965; 12(3):451-66. DOI: 10.1016/0012-1606(65)90009-6. View

20.

SCHREIER K, Strube C, PORATH U, PLUECKTHUN H . [Studies of the physiological development of the protein metabolism of liver tissue]. Biol Neonat. 1960; 2:178-92. View