Decreased Protein and Puromycinyl-peptide Degradation in Livers of Senescent Mice

Overview

Journal Biochem J

Specialty Biochemistry

Date 1982 Jan 15

PMID 7082317

Citations 7

Authors

L Lavie

A Z Reznick

D Gershon

Affiliations

Soon will be listed here.

Abstract

Liver protein-degradation rates were determined in young and old C57B1 mice by the method of Swick & Ip [(1974) J. Biol. Chem. 249, 6836-6841]. The results indicated a marked age-related increase in the half-lives of short-lived proteins in the nuclear, mitochondrial, lysosomal and 100000 g-supernatant cellular fractions and in total trichloroacetic acid-precipitable proteins. The efficiency of the degradation system in removing aberrant proteins from livers of young and old mice was tested. The time required for 50% disappearance of puromycinyl-peptides changed from about 20 min in 6-month-old mice to approx. 150 min in 24-month-old animals. These findings suggest that in old animals the proteolytic activity involved in degradation of aberrant proteins, and presumably of "native proteins, is markedly defective.

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