Effects of Three Different Ca(2+)-ATPase Inhibitors on Ca2+ Response and Leukotriene Release in RBL-2H3 Cells

Overview

Journal Inflamm Res

Specialties Allergy & Immunology
Pathology

Date 1996 Dec 1

PMID 8988402

Citations 1

Authors

R Akasaka

R Teshima

H IKEBUCHI

J Sawada

Affiliations

Soon will be listed here.

Abstract

The effects of three Ca(2+)-ATPase inhibitors, thapsigargin (TG), cyclopiazonic acid (CPA), and 2,5-di(tert-butyl)-1,4-hydroquinone (DTBHQ), on the Ca2+ response, degranulation, and leukotriene C4 (LTC4) release in RBL-2H3 cells were investigated. All three compounds elevated the intracellular free Ca2+ concentration ([Ca2+]i), and caused degranulation in the presence of 12-O-tetradecanoylphorbol-13-acetate (TPA), a protein kinase C activator. The dose-dependency of each compound in the Ca2+ response was in good agreement with that in degranulation. TG and CPA also caused the release of LTC4 in a dose-dependent manner, and this effect was unaffected by TPA or calphostin C, a selective PKC inhibitor. DTBHQ, however, did not induce LTC4 release, and rather inhibited the antigen-induced release of LTC4. These results suggest [1] that both degranulation and LTC4 release caused by these compounds are dependent on their [Ca2+]i increasing effect, [2] that degranulation and LTC4 release are mediated via independent pathways following the Ca2+ response, and [3] that DTBHQ additionally prevents the synthesis of LTC4 possibly by inhibition of 5-lipoxygenase.

Citing Articles

Modulation of basophils' degranulation and allergy-related enzymes by monomeric and dimeric naphthoquinones.

Pinho B, Sousa C, Valentao P, Oliveira J, Andrade P PLoS One. 2014; 9(2):e90122.

PMID: 24587235 PMC: 3938571. DOI: 10.1371/journal.pone.0090122.

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