The Effect of Nutrient Limitation on Styrene Metabolism in Pseudomonas Putida CA-3

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1996 Oct 1

PMID 8967774

Citations 6

Authors

K OConnor

W Duetz

B Wind

A D Dobson

Affiliations

Soon will be listed here.

Abstract

Styrene degradation in Pseudomonas putida CA-3 has previously been shown to be subject to catabolite repression in batch culture. We report here on the catabolite-repressing effects of succinate and glutamate and the effects of a limiting inorganic-nutrient concentration on the styrene degradation pathway of P. putida CA-3 in a chemostat culture at low growth rates (0.05 h-1). Oxidation of styrene and the presence of styrene oxide isomerase and phenylacetaldehyde dehydrogenase activities were used as a measure of the expression of the styrene degradation pathway. Both glutamate and succinate failed to repress the styrene degradation ability under growth conditions of carbon and energy limitation. Lower levels of enzyme activities of the styrene degradation pathway were seen in cells grown on styrene or phenylacetic acid (PAA) under conditions of both ammonia and sulfate limitation than were seen under carbon and energy limitation. Cells grown on PAA under continuous culture oxidize styrene and styrene oxide and possess styrene oxide isomerase and NAD(+)-dependent phenylacetaldehyde dehydrogenase activities. Catabolite repression of styrene metabolism was observed in cells grown on styrene or PAA in the presence of growth-saturating (nonlimiting) concentrations of succinate or glutamate under sulfate limitation.

Citing Articles

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Functional characterization of a StyS sensor kinase reveals distinct domains associated with intracellular and extracellular sensing of styrene in P. putida CA-3.

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Regulation of phenylacetic acid uptake is σ54 dependent in Pseudomonas putida CA-3.

O Leary N, O Mahony M, Dobson A BMC Microbiol. 2011; 11:229.

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Accumulation of polyhydroxyalkanoate from styrene and phenylacetic acid by Pseudomonas putida CA-3.

Ward P, de Roo G, OConnor K Appl Environ Microbiol. 2005; 71(4):2046-52.

PMID: 15812037 PMC: 1082534. DOI: 10.1128/AEM.71.4.2046-2052.2005.

p-Hydroxyphenylacetic Acid Metabolism in Pseudomonas putida F6.

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