Bacterial Degradation of Styrene Involving a Novel Flavin Adenine Dinucleotide-dependent Styrene Monooxygenase

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1990 May 1

PMID 2339888

Citations 73

Authors

S Hartmans

M J van der Werf

J A de Bont

Affiliations

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Abstract

By using styrene as the sole source of carbon and energy in concentrations of 10 to 500 microM, 14 strains of aerobic bacteria and two strains of fungi were isolated from various soil and water samples. In cell extracts of 11 of the bacterial isolates, a novel flavin adenine dinucleotide-requiring styrene monooxygenase activity that oxidized styrene to styrene oxide (phenyl oxirane) was detected. In one bacterial strain (S5), styrene metabolism was studied in more detail. In addition to styrene monooxygenase, cell extracts from strain S5 contained styrene oxide isomerase and phenylacetaldehyde dehydrogenase activities. A pathway for styrene degradation via styrene oxide and phenylacetaldehyde to phenylacetic acid is proposed.

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References

Hou C, Patel R, Laskin A, Barnabe N, Barist I . Epoxidation of short-chain alkenes by resting-cell suspensions of propane-grown bacteria. Appl Environ Microbiol. 1983; 46(1):171-7. PMC: 239284. DOI: 10.1128/aem.46.1.171-177.1983. View

Muller F . Flavin-dependent hydroxylases. Biochem Soc Trans. 1985; 13(2):443-7. DOI: 10.1042/bst0130443. View

Baggi L, Cianconi L, Venanzi L . [Ceramic onlays: light microscope study]. Attual Dent. 1990; 6(38):18-9. View

Colby J, Stirling D, Dalton H . The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds. Biochem J. 1977; 165(2):395-402. PMC: 1164912. DOI: 10.1042/bj1650395. View

Higgins I, HAMMOND R, Sariaslani F, Best D, Davies M, Tryhorn S . Biotransformation of hydrocarbons and related compounds by whole organism suspensions of methane-grown methylosinus trichosporium OB 3b. Biochem Biophys Res Commun. 1979; 89(2):671-7. DOI: 10.1016/0006-291x(79)90682-x. View