The PMT Gene Family: Protein O-glycosylation in Saccharomyces Cerevisiae is Vital

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1996 Nov 1

PMID 8918452

Citations 93

Authors

M Gentzsch

W Tanner

Affiliations

Soon will be listed here.

Abstract

The transfer of mannose to seryl and threonyl residues of secretory proteins is catalyzed by a family of protein mannosyltransferases coded for by seven genes (PMT1-7). Mannose dolichylphosphate is the sugar donor of the reaction, which is localized at the endoplasmic reticulum. By gene disruption and crosses all single, double and triple mutants of genes PMT1-4 were constructed. Two of the double and three of the triple mutants were not able to grow under normal conditions; three of these mutants could grow, however, when osmotically stabilized. The various mutants were extensively characterized concerning growth, morphology and their sensitivity to killer toxin K1, caffeine and calcofluor white. O-Mannosylation of gp115/Gas1p was affected only in pmt4 mutants, whereas glycosylation of chitinase was mainly affected in pmt1 and pmt2 mutants. The results show that protein O-glycosylation is essential for cell wall rigidity and cell integrity and that this protein modification, therefore, is vital for Saccharomyces cerevisiae.

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References

Jentoft N . Why are proteins O-glycosylated?. Trends Biochem Sci. 1990; 15(8):291-4. DOI: 10.1016/0968-0004(90)90014-3. View

Boone C, Sommer S, Hensel A, Bussey H . Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly. J Cell Biol. 1990; 110(5):1833-43. PMC: 2200168. DOI: 10.1083/jcb.110.5.1833. View

Tanner W . Protein O-glycosylation in Saccharomyces cerevisiae. Purification and characterization of the dolichyl-phosphate-D-mannose-protein O-D-mannosyltransferase. Eur J Biochem. 1991; 196(1):185-90. DOI: 10.1111/j.1432-1033.1991.tb15802.x. View

Sherman F, Hicks J . Micromanipulation and dissection of asci. Methods Enzymol. 1991; 194:21-37. DOI: 10.1016/0076-6879(91)94005-w. View

Sherman F . Getting started with yeast. Methods Enzymol. 1991; 194:3-21. DOI: 10.1016/0076-6879(91)94004-v. View

Vai M, Gatti E, Lacana E, Popolo L, Alberghina L . Isolation and deduced amino acid sequence of the gene encoding gp115, a yeast glycophospholipid-anchored protein containing a serine-rich region. J Biol Chem. 1991; 266(19):12242-8. View

Kuranda M, Robbins P . Chitinase is required for cell separation during growth of Saccharomyces cerevisiae. J Biol Chem. 1991; 266(29):19758-67. View

Levin D . Mutants in the S. cerevisiae PKC1 gene display a cell cycle-specific osmotic stability defect. J Cell Biol. 1992; 116(5):1221-9. PMC: 2289351. DOI: 10.1083/jcb.116.5.1221. View

Costigan C, Gehrung S, Snyder M . A synthetic lethal screen identifies SLK1, a novel protein kinase homolog implicated in yeast cell morphogenesis and cell growth. Mol Cell Biol. 1992; 12(3):1162-78. PMC: 369547. DOI: 10.1128/mcb.12.3.1162-1178.1992. View

10.

Hausler A, BALLOU L, Ballou C, Robbins P . Yeast glycoprotein biosynthesis: MNT1 encodes an alpha-1,2-mannosyltransferase involved in O-glycosylation. Proc Natl Acad Sci U S A. 1992; 89(15):6846-50. PMC: 49601. DOI: 10.1073/pnas.89.15.6846. View

11.

Paravicini G, Cooper M, Friedli L, Smith D, Carpentier J, Klig L . The osmotic integrity of the yeast cell requires a functional PKC1 gene product. Mol Cell Biol. 1992; 12(11):4896-905. PMC: 360422. DOI: 10.1128/mcb.12.11.4896-4905.1992. View

12.

Posas F, Casamayor A, Arino J . The PPZ protein phosphatases are involved in the maintenance of osmotic stability of yeast cells. FEBS Lett. 1993; 318(3):282-6. DOI: 10.1016/0014-5793(93)80529-4. View

13.

Lee K, Hines L, Levin D . A pair of functionally redundant yeast genes (PPZ1 and PPZ2) encoding type 1-related protein phosphatases function within the PKC1-mediated pathway. Mol Cell Biol. 1993; 13(9):5843-53. PMC: 360330. DOI: 10.1128/mcb.13.9.5843-5853.1993. View

14.

Immervoll T, Deutzmann R, Tanner W . PMT1, the gene for a key enzyme of protein O-glycosylation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1993; 90(17):8164-8. PMC: 47309. DOI: 10.1073/pnas.90.17.8164. View

15.

Montijn R, van Rinsum J, van Schagen F, Klis F . Glucomannoproteins in the cell wall of Saccharomyces cerevisiae contain a novel type of carbohydrate side chain. J Biol Chem. 1994; 269(30):19338-42. View

16.

Roth J, Wang Y, Eckhardt A, Hill R . Subcellular localization of the UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase-mediated O-glycosylation reaction in the submaxillary gland. Proc Natl Acad Sci U S A. 1994; 91(19):8935-9. PMC: 44721. DOI: 10.1073/pnas.91.19.8935. View

17.

Ram A, Wolters A, Ten Hoopen R, Klis F . A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to calcofluor white. Yeast. 1994; 10(8):1019-30. DOI: 10.1002/yea.320100804. View

18.

Lussier M, Gentzsch M, Sdicu A, Bussey H, Tanner W . Protein O-glycosylation in yeast. The PMT2 gene specifies a second protein O-mannosyltransferase that functions in addition to the PMT1-encoded activity. J Biol Chem. 1995; 270(6):2770-5. DOI: 10.1074/jbc.270.6.2770. View

19.

Gentzsch M, Tanner W . A new Dol-P-Man:protein O-D-mannosyltransferase activity from Saccharomyces cerevisiae. Glycobiology. 1995; 5(1):77-82. DOI: 10.1093/glycob/5.1.77. View

20.

Sorensen T, White T, Wandall H, Kristensen A, Roepstorff P, Clausen H . UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase. Identification and separation of two distinct transferase activities. J Biol Chem. 1995; 270(41):24166-73. DOI: 10.1074/jbc.270.41.24166. View