Yeast KRE Genes Provide Evidence for a Pathway of Cell Wall Beta-glucan Assembly

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1990 May 1

PMID 2186051

Citations 89

Authors

C Boone

S S Sommer

A Hensel

H Bussey

Affiliations

Soon will be listed here.

Abstract

The Saccharomyces cerevisiae KRE1 gene encodes a Ser/Thr-rich protein, that is directed into the yeast secretory pathway, where it is highly modified, probably through addition of O-linked mannose residues. Gene disruption of the KRE1 locus leads to a 40% reduced level of cell wall (1----6)-beta-glucan. Structural analysis of the (1----6)-beta-glucan fraction, isolated from a strain with a krel disruption mutation, showed that it had an altered structure with a smaller average polymer size. Mutations in two other loci, KRE5 and KRE6 also lead to a defect in cell wall (1----6)-beta-glucan production and appear to be epistatic to KRE1. These findings outline a possible pathway of assembly of yeast cell wall (1----6)-beta-glucan.

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