A Radioimmunoassay for the Quantitative Evaluation of Anti-human Acetylcholine Receptor Antibodies in Myasthenia Gravis

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1977 Jul 1

PMID 891031

Citations 21

Authors

V M Monnier

B W Fulpius

Affiliations

Soon will be listed here.

Abstract

A radioimmunoassay was developed for the quantitative evaluation of antibodies to the acetylcholine receptor in the serum of myasthenic patients. AcChR was extracted from human muscle. A detailed preparation of the 125I-labelled alpha-Bgt-AcChR complex used as antigen is reported. Usually, an average of 20 pmol were obtained form 100 g muscle. This preparation is stable for 1 month in presence of an inhibitor of proteolysis and sufficient for performing about fifteen assays. The labelled complex was incubated with increasing amounts of sera and precipitated with anti-human IgG serum. Titres were expressed in pmol 125I-labelled alpha-Bgt-AcChR complex precipitated per ml serum. Out of thirty-nine sera tested thirty-six had positive titres ranging from 0-1-46 pmol/ml. No anti-AcChR were detected in the sera from twenty-seven patients used as controls.

Citing Articles

Anti-acetylcholine receptor antibodies.

Vincent A, Newsom Davis J J Neurol Neurosurg Psychiatry. 1980; 43(7):590-600.

PMID: 7400823 PMC: 490626. DOI: 10.1136/jnnp.43.7.590.

Myasthenia gravis. Anti-acetylcholine receptor antibodies.

Cerrato D, Ariano C, La Mantia L, Fiacchino F, Sghirlanzoni A, Nespolo A Ital J Neurol Sci. 1981; 2(2):165-71.

PMID: 7333817 DOI: 10.1007/BF02335439.

A lipid-associated acetylcholine receptor as an antigen in diagnosis of myasthenia gravis.

Raimond F, Morel E Clin Exp Immunol. 1982; 47(2):345-50.

PMID: 7075027 PMC: 1536535.

Differences in the interaction of acetylcholine receptor antibodies with receptor from normal, denervated and myasthenic human muscle.

Lefvert A J Neurol Neurosurg Psychiatry. 1982; 45(1):70-3.

PMID: 7062074 PMC: 491268. DOI: 10.1136/jnnp.45.1.70.

Cellular and humoral immunity to acetylcholine receptor in myasthenia gravis.

Conti Tronconi B, Scotti A, Brigonzi A, Sher E, Fumagalli G, Peluchetti D Ital J Neurol Sci. 1982; 3(1):17-23.

PMID: 6979528 DOI: 10.1007/BF02043343.

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