Controlled Study of Cytolethal Distending Toxin-producing Escherichia Coli Infections in Bangladeshi Children

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1996 Mar 1

PMID 8904444

Citations 22

Authors

M J Albert

S M Faruque

A S Faruque

K A Bettelheim

P K Neogi

N A Bhuiyan

J B Kaper

Affiliations

Soon will be listed here.

Abstract

The role of cytolethal distending toxin (CDT)-producing Escherichia coli, a newly described category of E. coli, in the causation of diarrhea was studied by screening E. coli isolates from 546 children < 5 years of age with diarrhea and 215 matched controls without diarrhea by using a specific DNA probe. Although CDT-positive E. coli strains were isolated from more children with diarrhea than from healthy controls (3.1 versus 0.93%), this difference did not reach statistical significance (P = 0.082). All CDT-positive strains also possessed the virulence factors of enteropathogenic E. coli or enteroaggregative E. coli isolates.

Citing Articles

Cytolethal distending toxin-producing Escherichia coli clinical isolates from Mexican children harbor different cdt types causing CDT-induced epithelial pathological phenotypes.

Huerta-Cantillo J, Chavez-Duenas L, Zaidi M, Estrada-Garcia T, Navarro-Garcia F Med Microbiol Immunol. 2025; 214(1):7.

PMID: 39894890 PMC: 11788229. DOI: 10.1007/s00430-025-00816-4.

Epidemiology and Pathogenesis of Infections.

Shah M, Odoyo E, Ichinose Y Am J Trop Med Hyg. 2019; 101(2):290-293.

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Cytolethal distending toxin-producing strains causing severe diarrhoea in young Mexican children.

Meza-Segura M, Zaidi M, Maldonado-Puga S, Huerta-Cantillo J, Chavez-Duenas L, Navarro-Garcia F JMM Case Rep. 2017; 4(2):e005079.

PMID: 28348804 PMC: 5361634. DOI: 10.1099/jmmcr.0.005079.

The Cytolethal Distending Toxin Contributes to Microbial Virulence and Disease Pathogenesis by Acting As a Tri-Perditious Toxin.

Scuron M, Boesze-Battaglia K, Dlakic M, Shenker B Front Cell Infect Microbiol. 2016; 6:168.

PMID: 27995094 PMC: 5136569. DOI: 10.3389/fcimb.2016.00168.

Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan.

Hinenoya A, Shima K, Asakura M, Nishimura K, Tsukamoto T, Ooka T BMC Microbiol. 2014; 14:97.

PMID: 24742173 PMC: 4001111. DOI: 10.1186/1471-2180-14-97.

References

Evans D, Evans Jr D, Gorbach S . Identification of enterotoxigenic Escherichia coli and serum antitoxin activity by the vascular permeability factor assay. Infect Immun. 1973; 8(5):731-5. PMC: 422919. DOI: 10.1128/iai.8.5.731-735.1973. View

Hart C, Batt R, Saunders J . Diarrhoea caused by Escherichia coli. Ann Trop Paediatr. 1993; 13(2):121-31. DOI: 10.1080/02724936.1993.11747636. View

Stoll B, Glass R, Huq M, Khan M, Holt J, Banu H . Surveillance of patients attending a diarrhoeal disease hospital in Bangladesh. Br Med J (Clin Res Ed). 1982; 285(6349):1185-8. PMC: 1500105. DOI: 10.1136/bmj.285.6349.1185. View

Feinberg A, Vogelstein B . "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984; 137(1):266-7. DOI: 10.1016/0003-2697(84)90381-6. View

Bettelheim K, Thompson C . New method of serotyping Escherichia coli: implementation and verification. J Clin Microbiol. 1987; 25(5):781-6. PMC: 266088. DOI: 10.1128/jcm.25.5.781-786.1987. View

MANIAR A, Williams T, Hammond G . Detection of Clostridium difficile toxin in various tissue culture monolayers. J Clin Microbiol. 1987; 25(10):1999-2000. PMC: 269385. DOI: 10.1128/jcm.25.10.1999-2000.1987. View

Nataro J, Kaper J, Robins-Browne R, Prado V, Vial P, Levine M . Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatr Infect Dis J. 1987; 6(9):829-31. DOI: 10.1097/00006454-198709000-00008. View

Rahim Z, Kay B . Enrichment for Plesiomonas shigelloides from stools. J Clin Microbiol. 1988; 26(4):789-90. PMC: 266452. DOI: 10.1128/jcm.26.4.789-790.1988. View

Johnson W, Lior H . A new heat-labile cytolethal distending toxin (CLDT) produced by Escherichia coli isolates from clinical material. Microb Pathog. 1988; 4(2):103-13. DOI: 10.1016/0882-4010(88)90052-6. View

10.

Johnson W, Lior H . A new heat-labile cytolethal distending toxin (CLDT) produced by Campylobacter spp. Microb Pathog. 1988; 4(2):115-26. DOI: 10.1016/0882-4010(88)90053-8. View

11.

Knutton S, Baldwin T, Williams P, McNeish A . Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun. 1989; 57(4):1290-8. PMC: 313264. DOI: 10.1128/iai.57.4.1290-1298.1989. View

12.

Bouzari S, Varghese A . Cytolethal distending toxin (CLDT) production by enteropathogenic Escherichia coli (EPEC). FEMS Microbiol Lett. 1990; 59(1-2):193-8. DOI: 10.1016/0378-1097(90)90055-u. View

13.

Knutton S, SHAW R, Bhan M, Smith H, McConnell M, Cheasty T . Ability of enteroaggregative Escherichia coli strains to adhere in vitro to human intestinal mucosa. Infect Immun. 1992; 60(5):2083-91. PMC: 257118. DOI: 10.1128/iai.60.5.2083-2091.1992. View

14.

Bouzari S, Vatsala B, Varghese A . In vitro adherence property of cytolethal distending toxin (CLDT) producing EPEC strains and effect of the toxin on rabbit intestine. Microb Pathog. 1992; 12(2):153-7. DOI: 10.1016/0882-4010(92)90118-8. View

15.

Okuda J, Kurazono H, Takeda Y . Distribution of the cytolethal distending toxin A gene (cdtA) among species of Shigella and Vibrio, and cloning and sequencing of the cdt gene from Shigella dysenteriae. Microb Pathog. 1995; 18(3):167-72. DOI: 10.1016/s0882-4010(95)90022-5. View

16.

. Large epidemic of cholera-like disease in Bangladesh caused by Vibrio cholerae O139 synonym Bengal. Cholera Working Group, International Centre for Diarrhoeal Diseases Research, Bangladesh. Lancet. 1993; 342(8868):387-90. View

17.

Scott D, Kaper J . Cloning and sequencing of the genes encoding Escherichia coli cytolethal distending toxin. Infect Immun. 1994; 62(1):244-51. PMC: 186093. DOI: 10.1128/iai.62.1.244-251.1994. View

18.

Pickett C, Cottle D, Pesci E, Bikah G . Cloning, sequencing, and expression of the Escherichia coli cytolethal distending toxin genes. Infect Immun. 1994; 62(3):1046-51. PMC: 186222. DOI: 10.1128/iai.62.3.1046-1051.1994. View

19.

Sack R, Albert M, Alam K, Neogi P, Akbar M . Isolation of enterotoxigenic Bacteroides fragilis from Bangladeshi children with diarrhea: a controlled study. J Clin Microbiol. 1994; 32(4):960-3. PMC: 267162. DOI: 10.1128/jcm.32.4.960-963.1994. View

20.

Ratnayake S, Widmalm G, Weintraub A, Medina E . Structural studies of the Escherichia coli O90 O-antigen polysaccharide. Carbohydr Res. 1994; 263(2):209-15. DOI: 10.1016/0008-6215(94)00152-9. View