Cytolethal Distending Toxin Sequence and Activity in the Enterohepatic Pathogen Helicobacter Hepaticus

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1999 Dec 22

PMID 10603386

Citations 59

Authors

V B Young

K A Knox

D B Schauer

Affiliations

Soon will be listed here.

Abstract

Little is known about the molecular pathogenesis of hepatitis and enterocolitis caused by enterohepatic Helicobacter species. Sonicates of the murine pathogen Helicobacter hepaticus were found to cause progressive cell distension, accumulation of filamentous actin, and G(2)/M cell cycle arrest in HeLa cell monolayers. The genes encoding this cytotoxic activity were cloned from H. hepaticus. Three open reading frames with closest homology to cdtA, cdtB, and cdtC from Campylobacter jejuni were identified. Sonicates of a laboratory strain of Escherichia coli carrying the cloned cdtABC gene cluster from H. hepaticus reproduced the cytotoxic activities seen with sonicates of H. hepaticus. Cytolethal distending toxin activity is a potential virulence determinant of H. hepaticus that may play a role in the pathogenesis of Helicobacter-associated hepatitis and enterocolitis.

Citing Articles

Cytolethal distending toxin: from genotoxin to a potential biomarker and anti-tumor target.

Kailoo S, Shreya , Kumar Y World J Microbiol Biotechnol. 2021; 37(9):150.

PMID: 34379213 DOI: 10.1007/s11274-021-03117-z.

Intestinal Inflammation and Altered Gut Microbiota Associated with Inflammatory Bowel Disease Render Mice Susceptible to Clostridioides difficile Colonization and Infection.

Abernathy-Close L, Barron M, George J, Dieterle M, Vendrov K, Bergin I mBio. 2021; 12(3):e0273320.

PMID: 34126769 PMC: 8262858. DOI: 10.1128/mBio.02733-20.

The Majority of Typhoid Toxin-Positive Serovars Encode ArtB, an Alternate Binding Subunit.

Gaballa A, Cheng R, Harrand A, Cohn A, Wiedmann M mSphere. 2021; 6(1).

PMID: 33408236 PMC: 7845599. DOI: 10.1128/mSphere.01255-20.

Complete Genome Sequencing and Comparative Genomic Analysis of Isolated From the Wild Korean Striped Field Mouse ) for Potential Pathogenicity.

Kim J, Kim W, An J, Suh J, Seong J, Jeon B Front Pharmacol. 2018; 9:838.

PMID: 30131692 PMC: 6090156. DOI: 10.3389/fphar.2018.00838.

Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia.

Grillova L, Sedlacek I, Pachnikova G, Stankova E, Svec P, Holochova P J Vet Med Sci. 2017; 80(1):138-146.

PMID: 29249728 PMC: 5797873. DOI: 10.1292/jvms.17-0492.

References

Johnson W, Lior H . A new heat-labile cytolethal distending toxin (CLDT) produced by Escherichia coli isolates from clinical material. Microb Pathog. 1988; 4(2):103-13. DOI: 10.1016/0882-4010(88)90052-6. View

STEVENS M, Latimer J, Lumbley S, Ward C, Cope L, Lagergard T . Characterization of a Haemophilus ducreyi mutant deficient in expression of cytolethal distending toxin. Infect Immun. 1999; 67(8):3900-8. PMC: 96670. DOI: 10.1128/IAI.67.8.3900-3908.1999. View

Bouzari S, Varghese A . Cytolethal distending toxin (CLDT) production by enteropathogenic Escherichia coli (EPEC). FEMS Microbiol Lett. 1990; 59(1-2):193-8. DOI: 10.1016/0378-1097(90)90055-u. View

Young V, Falkow S, Schoolnik G . The invasin protein of Yersinia enterocolitica: internalization of invasin-bearing bacteria by eukaryotic cells is associated with reorganization of the cytoskeleton. J Cell Biol. 1992; 116(1):197-207. PMC: 2289272. DOI: 10.1083/jcb.116.1.197. View

Ward J, Fox J, Anver M, Haines D, George C, Collins Jr M . Chronic active hepatitis and associated liver tumors in mice caused by a persistent bacterial infection with a novel Helicobacter species. J Natl Cancer Inst. 1994; 86(16):1222-7. DOI: 10.1093/jnci/86.16.1222. View

Fox J, Dewhirst F, Tully J, Paster B, Yan L, Taylor N . Helicobacter hepaticus sp. nov., a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice. J Clin Microbiol. 1994; 32(5):1238-45. PMC: 263656. DOI: 10.1128/jcm.32.5.1238-1245.1994. View

Burnens A, Stanley J, Morgenstern R, Nicolet J . Gastroenteritis associated with Helicobacter pullorum. Lancet. 1994; 344(8936):1569-70. DOI: 10.1016/s0140-6736(94)90376-x. View

Stanley J, Linton D, Burnens A, Dewhirst F, On S, Porter A . Helicobacter pullorum sp. nov.-genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis. Microbiology (Reading). 1994; 140 ( Pt 12):3441-9. DOI: 10.1099/13500872-140-12-3441. View

Fox J, Yan L, Dewhirst F, Paster B, Shames B, Murphy J . Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice. J Clin Microbiol. 1995; 33(2):445-54. PMC: 227964. DOI: 10.1128/jcm.33.2.445-454.1995. View

10.

Taylor N, Fox J, Yan L . In-vitro hepatotoxic factor in Helicobacter hepaticus, H. pylori and other Helicobacter species. J Med Microbiol. 1995; 42(1):48-52. DOI: 10.1099/00222615-42-1-48. View

11.

Allos B, Blaser M . Campylobacter jejuni and the expanding spectrum of related infections. Clin Infect Dis. 1995; 20(5):1092-9; quiz 1100-1. DOI: 10.1093/clinids/20.5.1092. View

12.

Okuda J, Kurazono H, Takeda Y . Distribution of the cytolethal distending toxin A gene (cdtA) among species of Shigella and Vibrio, and cloning and sequencing of the cdt gene from Shigella dysenteriae. Microb Pathog. 1995; 18(3):167-72. DOI: 10.1016/s0882-4010(95)90022-5. View

13.

Fox J, Li X, Yan L, Cahill R, Hurley R, Lewis R . Chronic proliferative hepatitis in A/JCr mice associated with persistent Helicobacter hepaticus infection: a model of helicobacter-induced carcinogenesis. Infect Immun. 1996; 64(5):1548-58. PMC: 173960. DOI: 10.1128/iai.64.5.1548-1558.1996. View

14.

Ward J, Anver M, Haines D, Melhorn J, Gorelick P, Yan L . Inflammatory large bowel disease in immunodeficient mice naturally infected with Helicobacter hepaticus. Lab Anim Sci. 1996; 46(1):15-20. View

15.

Pickett C, Pesci E, Cottle D, Russell G, Erdem A, Zeytin H . Prevalence of cytolethal distending toxin production in Campylobacter jejuni and relatedness of Campylobacter sp. cdtB gene. Infect Immun. 1996; 64(6):2070-8. PMC: 174038. DOI: 10.1128/iai.64.6.2070-2078.1996. View

16.

Cover T . The vacuolating cytotoxin of Helicobacter pylori. Mol Microbiol. 1996; 20(2):241-6. DOI: 10.1111/j.1365-2958.1996.tb02612.x. View

17.

Fox J, Yan L, Shames B, Campbell J, Murphy J, Li X . Persistent hepatitis and enterocolitis in germfree mice infected with Helicobacter hepaticus. Infect Immun. 1996; 64(9):3673-81. PMC: 174280. DOI: 10.1128/iai.64.9.3673-3681.1996. View

18.

Albert M, Faruque S, Faruque A, Bettelheim K, Neogi P, Bhuiyan N . Controlled study of cytolethal distending toxin-producing Escherichia coli infections in Bangladeshi children. J Clin Microbiol. 1996; 34(3):717-9. PMC: 228876. DOI: 10.1128/jcm.34.3.717-719.1996. View

19.

Censini S, Lange C, Xiang Z, Crabtree J, Ghiara P, Borodovsky M . cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc Natl Acad Sci U S A. 1996; 93(25):14648-53. PMC: 26189. DOI: 10.1073/pnas.93.25.14648. View

20.

Okuda J, Fukumoto M, Takeda Y, Nishibuchi M . Examination of diarrheagenicity of cytolethal distending toxin: suckling mouse response to the products of the cdtABC genes of Shigella dysenteriae. Infect Immun. 1997; 65(2):428-33. PMC: 174612. DOI: 10.1128/iai.65.2.428-433.1997. View