Isotachophoretic Analysis of Mandelic Acid, Phenylglyoxylic Acid, Hippuric Acid and Methylhippuric Acid in Urine After Occupational Exposure to Styrene, Toluene And/or Xylene

A simple, rapid and sensitive analytical method has been developed for the determination of phenylglyoxylic acid, mandelic acid, hippuric acid and methylhippuric acid; 0-, m- and p-methylhippuric acids are partly separated. These compounds are found as metabolites after occupational exposure to styrene, toluene and xylene. The method has been applied successfully to samples extracted from human urine by diethyl ether. The method can be used to accurately and simultaneously determine as little as 0.5 nmole of all of these acids in less than 20 min.