Immunogold Labelling of Neuroendocrine Peptides with Special Reference to Antibody Specificity and Multiple Staining Techniques

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 1996 Jul 1

PMID 8858369

Citations 2

Authors

L I Larsson

Affiliations

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Abstract

Immunogold methods have been very important for research on the neuroendocrine system. The compatibility of immunogold probes with optimal contrasting for electron microscopy has made localizations of neuroendocrine peptides to different subtypes of secretory organelles possible and, currently, methods using covalent attachment of nanogold particles to antibodies and neuropeptide ligands hold promise for immunocytochemistry, receptor localizations and in situ hybridizations. Multihormonal phenotypes are a hallmark of both the developing and mature neuroendocrine system. The possibility to localize multiple coexisting messengers by multilabelling immunogold methods is emphasized, and different methods for achieving this are discussed. The most difficult part of immunocytochemistry involves definitions and interpretations of specificity, and a number of limitations and control procedures are discussed.

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References

Timms B . Postembedding immunogold labeling for electron microscopy using "LR White" resin. Am J Anat. 1986; 175(2-3):267-75. DOI: 10.1002/aja.1001750211. View

Scopsi L, Larsson L . Colloidal gold probes in immunocytochemistry. An optimization of their application in light microscopy by use of silver intensification procedures. Med Biol. 1986; 64(2-3):139-45. View

Yokota S . Effect of particle size on labeling density for catalase in protein A-gold immunocytochemistry. J Histochem Cytochem. 1988; 36(1):107-9. DOI: 10.1177/36.1.3335766. View

Pearse A . Common cytochemical and ultrastructural characteristics of cells producing polypeptide hormones (the APUD series) and their relevance to thyroid and ultimobranchial C cells and calcitonin. Proc R Soc Lond B Biol Sci. 1968; 170(1018):71-80. DOI: 10.1098/rspb.1968.0025. View

Larsson L . Simultaneous ultrastructural demonstration of multiple peptides in endocrine cells by a novel immunocytochemical method. Nature. 1979; 282(5740):743-6. DOI: 10.1038/282743a0. View

Alexander R, Isaacs W, Barrack E . Immunogold probes for electron microscopy: evaluation of staining by fluorescence microscopy. J Histochem Cytochem. 1985; 33(10):995-1000. DOI: 10.1177/33.10.2413103. View

Bienz K, Egger D, Pasamontes L . Electron microscopic immunocytochemistry. Silver enhancement of colloidal gold marker allows double labeling with the same primary antibody. J Histochem Cytochem. 1986; 34(10):1337-42. DOI: 10.1177/34.10.3745912. View

Baldwin G . The role of gastrin and cholecystokinin in normal and neoplastic gastrointestinal growth. J Gastroenterol Hepatol. 1995; 10(2):215-32. DOI: 10.1111/j.1440-1746.1995.tb01083.x. View

Roth J, Bendayan M, Carlemalm E, Villiger W, Garavito M . Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem. 1981; 29(5):663-71. DOI: 10.1177/29.5.6166664. View

10.

Larsson L, Jorgensen L . Ultrastructural and cytochemical studies on the cytodifferentiation of duodenal endocrine cells. Cell Tissue Res. 1978; 194(1):79-102. DOI: 10.1007/BF00209235. View

11.

van Dongen J, Hooijkaas H, Comans-Bitter W, Benne K, Van Os T, de Josselin de Jong J . Triple immunological staining with colloidal gold, fluorescein and rhodamine as labels. J Immunol Methods. 1985; 80(1):1-6. DOI: 10.1016/0022-1759(85)90157-7. View

12.

Handley D, Chien S . Colloidal gold: a pluripotent receptor probe. Proc Soc Exp Biol Med. 1983; 174(1):1-11. DOI: 10.3181/00379727-174-41697. View

13.

Herrera P, Huarte J, Sanvito F, Meda P, Orci L, Vassalli J . Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene. Development. 1991; 113(4):1257-65. DOI: 10.1242/dev.113.4.1257. View

14.

Roth J . The preparation of protein A-gold complexes with 3 nm and 15nm gold particles and their use in labelling multiple antigens on ultra-thin sections. Histochem J. 1982; 14(5):791-801. DOI: 10.1007/BF01033628. View

15.

Geuze H, Slot J, van der Ley P, Scheffer R . Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections. J Cell Biol. 1981; 89(3):653-65. PMC: 2111804. DOI: 10.1083/jcb.89.3.653. View

16.

Roth K, Gordon J . Spatial differentiation of the intestinal epithelium: analysis of enteroendocrine cells containing immunoreactive serotonin, secretin, and substance P in normal and transgenic mice. Proc Natl Acad Sci U S A. 1990; 87(16):6408-12. PMC: 54543. DOI: 10.1073/pnas.87.16.6408. View

17.

Alpert S, Hanahan D, Teitelman G . Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons. Cell. 1988; 53(2):295-308. DOI: 10.1016/0092-8674(88)90391-1. View

18.

Armbruster B, Garavito R, Kellenberger E . Dehydration and embedding temperatures affect the antigenic specificity of tubulin and immunolabeling by the protein A-colloidal gold technique. J Histochem Cytochem. 1983; 31(12):1380-4. DOI: 10.1177/31.12.6195215. View

19.

Roth J, Bendayan M, Orci L . Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem. 1978; 26(12):1074-81. DOI: 10.1177/26.12.366014. View

20.

Jackerott M, Oster A, Larsson L . PYY in developing murine islet cells: comparisons to development of islet hormones, NPY, and BrdU incorporation. J Histochem Cytochem. 1996; 44(8):809-17. DOI: 10.1177/44.8.8756753. View