Triple Immunological Staining with Colloidal Gold, Fluorescein and Rhodamine As Labels

Overview

Journal J Immunol Methods

Publisher Elsevier

Specialties Allergy & Immunology
Pathology

Date 1985 Jun 12

PMID 2409172

Citations 3

Authors

J J van Dongen

H Hooijkaas

W M Comans-Bitter

K Benne

T M Van Os

J de Josselin de Jong

Affiliations

Soon will be listed here.

Abstract

Colloidal gold particles are detectable by light microscopy with polarized light in the same epi-illumination system as for fluorescence microscopy. Colloidal gold particles can thus be used in combination with a fluorochrome for the combined immunological detection of surface membrane and cytoplasmic markers. We analyzed human bone marrow cells by a triple immunological staining for 3 different markers, using colloidal gold, fluorescein and rhodamine as labels. Our results demonstrate that such a triple immunological staining provides a powerful tool for study of the heterogeneity of small cell populations.

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