The Prevention of Repeat-associated Deletions in Saccharomyces Cerevisiae by Mismatch Repair Depends on Size and Origin of Deletions

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1996 Aug 1

PMID 8844147

Citations 32

Authors

H T Tran

D A Gordenin

M A Resnick

Affiliations

Soon will be listed here.

Abstract

We have investigated the effects of mismatch repair on 1-to 61-bp deletions in the yeast Saccharomyces cerevisiae. The deletions are likely to involve unpaired loop intermediates resulting from DNA polymerase slippage. The mutator effects of mutations in the DNA polymerase delta (POL3) gene and the recombinational repair RAD52 gene were studied in combination with mismatch repair defects. The pol3-t mutation increased up to 1000-fold the rate of extended (7-61 bp) but not of 1-bp deletions. In a rad52 null mutant only the 1-bp deletions were increased (12-fold). The mismatch repair mutations pms1, msh2 and msh3 did not affect 31- and 61-bp deletions in the pol3-t but increased the rates of 7- and 1-bp deletions. We propose that loops less than or equal to seven bases generated during replication are subject to mismatch repair by the PMS1, MSH2, MSH3 system and that in cannot act on loops > or = 31 bases. In contrast to the pol3-t, the enhancement of 1-bp deletions in a rad52 mutant is not altered by a pms1 mutation. Thus, mismatch repair appears to be specific to errors of DNA synthesis generated during semiconservative replication.

Citing Articles

Transcription-coupled repair and mismatch repair contribute towards preserving genome integrity at mononucleotide repeat tracts.

Georgakopoulos-Soares I, Koh G, Momen S, Jiricny J, Hemberg M, Nik-Zainal S Nat Commun. 2020; 11(1):1980.

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Defects in the GINS complex increase the instability of repetitive sequences via a recombination-dependent mechanism.

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Recovery of arrested replication forks by homologous recombination is error-prone.

Iraqui I, Chekkal Y, Jmari N, Pietrobon V, Freon K, Costes A PLoS Genet. 2012; 8(10):e1002976.

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Mismatch repair-independent increase in spontaneous mutagenesis in yeast lacking non-essential subunits of DNA polymerase ε.

Aksenova A, Volkov K, Maceluch J, Pursell Z, Rogozin I, Kunkel T PLoS Genet. 2010; 6(11):e1001209.

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Mutator phenotypes due to DNA replication infidelity.

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